Crystallization (Comment) | Organism |
---|---|
purified recombinant apo MAP2K4-KD or in complex with ANP, 15 mg/ml protein in a solution containing 20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 10% glycerol and 10 mM DTT for the apoenzyme, and 2 mM ANP and MgCl2 additional for the npMAP2K4/ANP complex, mixing with reservoir solution containing 0.2 M ammonium acetate, 22-25% w/v PEG 3350 and 0.1 M HEPESNaOH, pH 7.0, 20°C, X-ray diffraction structure determination and analysis at 3.5 A resolution | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37950 | - |
- |
Homo sapiens |
39000 | - |
- |
Homo sapiens |
40000 | - |
- |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P45985 | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 37419 , apo npMAP2K4, sequence calculation, x * 37950, npMAP2K4/ANP complex, sequence calculation, x * 39000 , apo npMAP2K4, light scattering, x * 40000, npMAP2K4/ANP complex, light scattering | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
MAP2K4 | - |
Homo sapiens |
mitogen-activated protein kinase kinase 4 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | upon ATP-binding, the transient conformation adopted the configuration of typical kinase folding. In the absence of ATP-binding, the transient state of apo npMAP2K4 may shift to a state of aggregation via non-particular hydrophobic interactions as a result of the exposed hydrophobic residues | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | the transient state of apo npMAP2K4 exists between the canonical kinase fold and the aggregation state. The ATP molecule under physiological conditions allows the transient conformation to rapidly assume the canonical kinase fold but the depletion of ATP under conditions of low levels of bioactivity perhaps enhances the transition to the aggregation state | Homo sapiens |
physiological function | mitogen-activated protein kinase kinase 4 (MAP2K4) plays a crucial role in the stress-activated signal cascade and is enzymatically regulated by ligand or substrate binding, and/or post-translational modification | Homo sapiens |