Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | MKK3 expression is induced upon Pseudomonas syringae infection | Arabidopsis thaliana |
Application | Comment | Organism |
---|---|---|
additional information | MKK3 pathway plays a role in pathogen defense, MKK3 is an upstream activator of the group C MAPKs MPK1, MPK2, MPK7, and MPK14. MKK3 shows the strongest interaction with MPK7, it phosphorylates MPK7. H2O2, but not flg22, activates MPK7 in an MKK3-mediated manner, MKK3 and MPK7 are part of a signaling pathway that regulates PR1 expression | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
construction of a 35S:MKK3-WT:myc expression cassette, either myc epitope-tagged wild-type MKK3 or constitutively active MKK3 are transiently expressed in Arabidopsis thaliana protoplasts, co-overexpression of MKK3 with MKK7 in ProPR1:GUS transgenic cell line, interaction analysis of MKK3 with group C MAPK kinases by yeast two-hybrid system, MKK3 and MPK7 induce the PR1 promoter, overview | Arabidopsis thaliana |
open reading frames of MKK3 cloned into the plant expression vector pRT100 and fused at their C-terminal end to a c-myc epitope. Arabidopsis protoplast transient expression | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mkk3 knockout plants, growth of virulent Pseudomonas syringae is increased | Arabidopsis thaliana |
additional information | transgenic ProMKK3:GUS lines show basal expression in vascular tissues that is strongly induced by Pseudomonas syringae pv tomato strain DC3000 infection but not by abiotic stresses. The growth of virulent Pst DC3000 is increased in mkk3 knockout plants and decreased in MKK3-overexpressing plants, MKK3 overexpression lines show increased expression of several PR genes, phenotypes, overview | Arabidopsis thaliana |
S235E/T241E | constitutively active kinase, MKK3-overexpressing plants, show significantly less Pseudomonas syringae growth compared with wild-type plants at 48 and 72 h after inoculation | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
protoplast | - |
Arabidopsis thaliana | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + MPK-7 | Arabidopsis thaliana | MKK3 interacts with MPK7 in planta | ADP + phospho-MPK-7 | - |
? | |
ATP + MPK-7 | Arabidopsis thaliana Col-0 | MKK3 interacts with MPK7 in planta | ADP + phospho-MPK-7 | - |
? | |
additional information | Arabidopsis thaliana | MKK3 positively regulates PR gene expression and plays a role in defense against Pst DC3000. MKK3 is an upstream activator of the group C MAPKs MPK1, MPK2, MPK7, and MPK14, overview | ? | - |
? | |
additional information | Arabidopsis thaliana Col-0 | MKK3 positively regulates PR gene expression and plays a role in defense against Pst DC3000. MKK3 is an upstream activator of the group C MAPKs MPK1, MPK2, MPK7, and MPK14, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
Col-0 | - |
Arabidopsis thaliana | - |
the genome contains genes encoding 10 MAPK kinases | - |
Arabidopsis thaliana Col-0 | - |
Col-0 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Arabidopsis thaliana | - |
vascular tissue | transgenic ProMKK3:GUS lines show basal expression that is strongly induced by Pseudomonas syringae pv tomato strain DC3000 infection but not by abiotic stresses | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + MPK-7 | - |
Arabidopsis thaliana | ADP + phospho-MPK-7 | - |
? | |
ATP + MPK-7 | MKK3 interacts with MPK7 in planta | Arabidopsis thaliana | ADP + phospho-MPK-7 | - |
? | |
ATP + MPK-7 | - |
Arabidopsis thaliana Col-0 | ADP + phospho-MPK-7 | - |
? | |
ATP + MPK-7 | MKK3 interacts with MPK7 in planta | Arabidopsis thaliana Col-0 | ADP + phospho-MPK-7 | - |
? | |
additional information | MKK3 positively regulates PR gene expression and plays a role in defense against Pst DC3000. MKK3 is an upstream activator of the group C MAPKs MPK1, MPK2, MPK7, and MPK14, overview | Arabidopsis thaliana | ? | - |
? | |
additional information | MKK3 positively regulates PR gene expression and plays a role in defense against Pst DC3000. MKK3 is an upstream activator of the group C MAPKs MPK1, MPK2, MPK7, and MPK14, overview | Arabidopsis thaliana Col-0 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MAPK kinase | - |
Arabidopsis thaliana |
MAPKK | - |
Arabidopsis thaliana |
MKK3 | - |
Arabidopsis thaliana |
More | MKK3 is a group B MAPK kinase | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Arabidopsis thaliana |