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Literature summary for 2.7.12.2 extracted from

  • Doczi, R.; Brader, G.; Pettko-Szandtner, A.; Rajh, I.; Djamei, A.; Pitzschke, A.; Teige, M.; Hirt, H.
    The Arabidopsis mitogen-activated protein kinase kinase MKK3 is upstream of group C mitogen-activated protein kinases and participates in pathogen signaling (2007), Plant Cell, 19, 3266-3279.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information MKK3 expression is induced upon Pseudomonas syringae infection Arabidopsis thaliana

Application

Application Comment Organism
additional information MKK3 pathway plays a role in pathogen defense, MKK3 is an upstream activator of the group C MAPKs MPK1, MPK2, MPK7, and MPK14. MKK3 shows the strongest interaction with MPK7, it phosphorylates MPK7. H2O2, but not flg22, activates MPK7 in an MKK3-mediated manner, MKK3 and MPK7 are part of a signaling pathway that regulates PR1 expression Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
construction of a 35S:MKK3-WT:myc expression cassette, either myc epitope-tagged wild-type MKK3 or constitutively active MKK3 are transiently expressed in Arabidopsis thaliana protoplasts, co-overexpression of MKK3 with MKK7 in ProPR1:GUS transgenic cell line, interaction analysis of MKK3 with group C MAPK kinases by yeast two-hybrid system, MKK3 and MPK7 induce the PR1 promoter, overview Arabidopsis thaliana
open reading frames of MKK3 cloned into the plant expression vector pRT100 and fused at their C-terminal end to a c-myc epitope. Arabidopsis protoplast transient expression Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information mkk3 knockout plants, growth of virulent Pseudomonas syringae is increased Arabidopsis thaliana
additional information transgenic ProMKK3:GUS lines show basal expression in vascular tissues that is strongly induced by Pseudomonas syringae pv tomato strain DC3000 infection but not by abiotic stresses. The growth of virulent Pst DC3000 is increased in mkk3 knockout plants and decreased in MKK3-overexpressing plants, MKK3 overexpression lines show increased expression of several PR genes, phenotypes, overview Arabidopsis thaliana
S235E/T241E constitutively active kinase, MKK3-overexpressing plants, show significantly less Pseudomonas syringae growth compared with wild-type plants at 48 and 72 h after inoculation Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
protoplast
-
Arabidopsis thaliana
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + MPK-7 Arabidopsis thaliana MKK3 interacts with MPK7 in planta ADP + phospho-MPK-7
-
?
ATP + MPK-7 Arabidopsis thaliana Col-0 MKK3 interacts with MPK7 in planta ADP + phospho-MPK-7
-
?
additional information Arabidopsis thaliana MKK3 positively regulates PR gene expression and plays a role in defense against Pst DC3000. MKK3 is an upstream activator of the group C MAPKs MPK1, MPK2, MPK7, and MPK14, overview ?
-
?
additional information Arabidopsis thaliana Col-0 MKK3 positively regulates PR gene expression and plays a role in defense against Pst DC3000. MKK3 is an upstream activator of the group C MAPKs MPK1, MPK2, MPK7, and MPK14, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
Col-0
-
Arabidopsis thaliana
-
the genome contains genes encoding 10 MAPK kinases
-
Arabidopsis thaliana Col-0
-
Col-0
-

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
Arabidopsis thaliana
-
vascular tissue transgenic ProMKK3:GUS lines show basal expression that is strongly induced by Pseudomonas syringae pv tomato strain DC3000 infection but not by abiotic stresses Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + MPK-7
-
Arabidopsis thaliana ADP + phospho-MPK-7
-
?
ATP + MPK-7 MKK3 interacts with MPK7 in planta Arabidopsis thaliana ADP + phospho-MPK-7
-
?
ATP + MPK-7
-
Arabidopsis thaliana Col-0 ADP + phospho-MPK-7
-
?
ATP + MPK-7 MKK3 interacts with MPK7 in planta Arabidopsis thaliana Col-0 ADP + phospho-MPK-7
-
?
additional information MKK3 positively regulates PR gene expression and plays a role in defense against Pst DC3000. MKK3 is an upstream activator of the group C MAPKs MPK1, MPK2, MPK7, and MPK14, overview Arabidopsis thaliana ?
-
?
additional information MKK3 positively regulates PR gene expression and plays a role in defense against Pst DC3000. MKK3 is an upstream activator of the group C MAPKs MPK1, MPK2, MPK7, and MPK14, overview Arabidopsis thaliana Col-0 ?
-
?

Synonyms

Synonyms Comment Organism
MAPK kinase
-
Arabidopsis thaliana
MAPKK
-
Arabidopsis thaliana
MKK3
-
Arabidopsis thaliana
More MKK3 is a group B MAPK kinase Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP
-
Arabidopsis thaliana