Application | Comment | Organism |
---|---|---|
additional information | MEK1 is an important regulator of SMAD3 expression | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
A549 cells transfected with wild-type or constitutively active MEK1 cDNA, both MEK1 expressions lead to increased SMAD3 expression and ERK phosphorylation | Homo sapiens |
MEK1, sequence comparison | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | positive regulation of SMAD3 expression is demonstrated by expression of a constitutively active ca-MEK1 construct, where the presence of ca-MEK1 results in increased SMAD3 protein expression | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
PD98059 | inhibition of MEK1 with either PD98059 results in a substantial dose-dependent inhibition of SMAD3 promoter activity, reduced SMAD3 protein expression in A549 cells and airway smooth muscle cells after treatment with MEK1 inhibitors; substantial dose-dependent inhibition of SMAD3 promoter activity, 5.8fold reduction, MEK1 inhibition of SMAD3 promoter function is primarily mediated through an Sp1-dependent mechanism | Homo sapiens | |
U0126 | inhibition of MEK1 with either UO126 results in a substantial dose-dependent inhibition of SMAD3 promoter activity, reduced SMAD3 protein expression in A549 cells and airway smooth muscle cells after treatment with MEK1 inhibitors; substantial dose-dependent inhibition of SMAD3 promoter activity, 2.3fold reduction, MEK1 inhibition of SMAD3 promoter function is primarily mediated through an Sp1-dependent mechanism | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Smad3 | Homo sapiens | mitogen-activated protein kinase kinase-1 regulates SMAD3 expression in epithelial and smooth muscle cells, which is stimulated by TGFbeta-1, SMAD3 is a transcription factor that mediates TGF-?1 signaling and is important in many of the cellular processes that regulate fibrosis and inflammation, overview | ADP + phosphorylated Smad3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A-549 cell | - |
Homo sapiens | - |
A-549 cell | human alveolar Type II epithelial adenocarcinoma cell line | Homo sapiens | - |
adenocarcinoma cell line | epithelium | Homo sapiens | - |
airway smooth muscle | - |
Homo sapiens | - |
smooth muscle cell | airway | Homo sapiens | - |
smooth muscle cell | bronchial airway | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Smad3 | - |
Homo sapiens | ADP + phosphorylated Smad3 | - |
? | |
ATP + Smad3 | mitogen-activated protein kinase kinase-1 regulates SMAD3 expression in epithelial and smooth muscle cells, which is stimulated by TGFbeta-1, SMAD3 is a transcription factor that mediates TGF-?1 signaling and is important in many of the cellular processes that regulate fibrosis and inflammation, overview | Homo sapiens | ADP + phosphorylated Smad3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MEK1 | - |
Homo sapiens |
mitogen-activated protein kinase kinase-1 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |