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Literature summary for 2.7.12.2 extracted from

  • Ross, K.R.; Corey, D.A.; Dunn, J.M.; Kelley, T.J.
    SMAD3 expression is regulated by mitogen-activated protein kinase kinase-1 in epithelial and smooth muscle cells (2007), Cell. Signal., 19, 923-931.
    View publication on PubMed

Application

Application Comment Organism
additional information MEK1 is an important regulator of SMAD3 expression Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
A549 cells transfected with wild-type or constitutively active MEK1 cDNA, both MEK1 expressions lead to increased SMAD3 expression and ERK phosphorylation Homo sapiens
MEK1, sequence comparison Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information positive regulation of SMAD3 expression is demonstrated by expression of a constitutively active ca-MEK1 construct, where the presence of ca-MEK1 results in increased SMAD3 protein expression Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
PD98059 inhibition of MEK1 with either PD98059 results in a substantial dose-dependent inhibition of SMAD3 promoter activity, reduced SMAD3 protein expression in A549 cells and airway smooth muscle cells after treatment with MEK1 inhibitors; substantial dose-dependent inhibition of SMAD3 promoter activity, 5.8fold reduction, MEK1 inhibition of SMAD3 promoter function is primarily mediated through an Sp1-dependent mechanism Homo sapiens
U0126 inhibition of MEK1 with either UO126 results in a substantial dose-dependent inhibition of SMAD3 promoter activity, reduced SMAD3 protein expression in A549 cells and airway smooth muscle cells after treatment with MEK1 inhibitors; substantial dose-dependent inhibition of SMAD3 promoter activity, 2.3fold reduction, MEK1 inhibition of SMAD3 promoter function is primarily mediated through an Sp1-dependent mechanism Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + Smad3 Homo sapiens mitogen-activated protein kinase kinase-1 regulates SMAD3 expression in epithelial and smooth muscle cells, which is stimulated by TGFbeta-1, SMAD3 is a transcription factor that mediates TGF-?1 signaling and is important in many of the cellular processes that regulate fibrosis and inflammation, overview ADP + phosphorylated Smad3
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
A-549 cell
-
Homo sapiens
-
A-549 cell human alveolar Type II epithelial adenocarcinoma cell line Homo sapiens
-
adenocarcinoma cell line epithelium Homo sapiens
-
airway smooth muscle
-
Homo sapiens
-
smooth muscle cell airway Homo sapiens
-
smooth muscle cell bronchial airway Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + Smad3
-
Homo sapiens ADP + phosphorylated Smad3
-
?
ATP + Smad3 mitogen-activated protein kinase kinase-1 regulates SMAD3 expression in epithelial and smooth muscle cells, which is stimulated by TGFbeta-1, SMAD3 is a transcription factor that mediates TGF-?1 signaling and is important in many of the cellular processes that regulate fibrosis and inflammation, overview Homo sapiens ADP + phosphorylated Smad3
-
?

Synonyms

Synonyms Comment Organism
MEK1
-
Homo sapiens
mitogen-activated protein kinase kinase-1
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens