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Literature summary for 2.7.12.2 extracted from
- LmxPK4, a mitogen-activated protein kinase kinase homologue of Leishmania mexicana with a potential role in parasite differentiation (2005), Mol. Microbiol., 56, 1169-1182.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, expression of wild-type and mutant GST-fusion enzymes in Escherichia coli, expression of a GFP-tagged enzyme in the null mutant strain, complementation of the null mutant strain by expression of the enzyme from plasmid or by reintegration of the wild-type gene into the genomic DNA locus | Leishmania mexicana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K102R | site-directed mutagenesis, kinase inactive mutant, but slight autophosphorylation activity, the mutant strain shows a phenotype with a proliferation defect after infection of human macrophages and no or delayed lesion development in mice | Leishmania mexicana |
| additional information | construction of deletion/disruption, null, or loss-of-function mutants, which all show a phenotype with a proliferation defect after infection of human macrophages and no or delayed lesion development in mice, gene replacement method | Leishmania mexicana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Leishmania mexicana | 5829 | - |
| flagellum | of promastigotes | Leishmania mexicana | - |
- |
| nucleus | - |
Leishmania mexicana | 5634 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38900 | - |
x * 38900, sequence calculation, x * 39000, recombinant enzyme, SDS-PAGE | Leishmania mexicana |
| 39000 | - |
x * 38900, sequence calculation, x * 39000, recombinant enzyme, SDS-PAGE | Leishmania mexicana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + MAP kinase | Leishmania mexicana | the enzyme is involved in the mitogen-activated protein kinase signaling pathway important for e.g. flagellar length control, the enzyme is involved in differentiation of the parasite and is important for virulence and infection of human peritoneal macrophages | ADP + phosphorylated MAP kinase | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leishmania mexicana | Q9GRT1 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | the enzyme performs autophosphorylation | Leishmania mexicana |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant GST-fusion enzymes from Escherichia coli by glutathione affinity chromatography | Leishmania mexicana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | the enzyme is developmentally regulated expressed, no expression in the amastigote stage in human host cells | Leishmania mexicana | - |
| promastigote | and during differentiation to amastigote | Leishmania mexicana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + MAP kinase | the enzyme is involved in the mitogen-activated protein kinase signaling pathway important for e.g. flagellar length control, the enzyme is involved in differentiation of the parasite and is important for virulence and infection of human peritoneal macrophages | Leishmania mexicana | ADP + phosphorylated MAP kinase | - |
? | |
| ATP + MAP kinase | docking site for the substrate is located close to the N-terminal Lys4 and consists of 2 basic amino acid residues separated by a spacer of 2-6 variable residues from an L/I/V-X-L/I/V sequence | Leishmania mexicana | ADP + phosphorylated MAP kinase | - |
? | |
| ATP + myelin basic protein | - |
Leishmania mexicana | ADP + phosphorylated myelin basic protein | - |
? | |
| additional information | the enzyme performs autophosphorylation | Leishmania mexicana | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 38900, sequence calculation, x * 39000, recombinant enzyme, SDS-PAGE | Leishmania mexicana |
| More | the enzyme contains a protein kinase domain, residues L73-I331, with 11 highly conserved subdomains, and a subdomain II with an invariable lysine residue responsible for ATP binding | Leishmania mexicana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MAP kinase kinase homologue | - |
Leishmania mexicana |
| mitogen-activated protein kinase kinase 4 | - |
Leishmania mexicana |
| mitogen-activated protein kinase kinase homologue | - |
Leishmania mexicana |
| MKK homologue | - |
Leishmania mexicana |
| PK4 | - |
Leishmania mexicana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Leishmania mexicana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | subdomain II with an invariable lysine residue is responsible for ATP binding | Leishmania mexicana | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Leishmania mexicana | sequence calculation | - |
7.4 |
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