Any feedback?
Literature summary for 2.7.11.4 extracted from
- Branched-chain alpha-keto acid dehydrogenase and its kinase from rabbit liver and heart (1988), Methods Enzymol., 166, 313-320.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-Chloroisohexanoate | - |
Bos taurus |  |
| 2-Chloroisohexanoate | - |
Oryctolagus cuniculus | |
| 2-Chloroisohexanoate | - |
Rattus norvegicus | |
| 2-oxo-3-methylpentanoate | - |
Oryctolagus cuniculus | |
| 2-oxo-3-methylpentanoate | - |
Rattus norvegicus | |
| 2-oxobutanoate | - |
Oryctolagus cuniculus | |
| 2-oxohexanedioate | - |
Bos taurus | |
| 2-oxohexanedioate | - |
Oryctolagus cuniculus | |
| 2-oxohexanedioate | - |
Rattus norvegicus | |
| 2-Oxohexanoate | - |
Oryctolagus cuniculus | |
| 2-oxoisocaproate | - |
Bos taurus | |
| 2-oxoisocaproate | - |
Oryctolagus cuniculus | |
| 2-oxoisocaproate | - |
Rattus norvegicus | |
| 2-oxoisopentanoate | - |
Oryctolagus cuniculus | |
| 2-oxoisopentanoate | - |
Rattus norvegicus | |
| 2-oxopentanoate | - |
Bos taurus | |
| 2-oxopentanoate | - |
Oryctolagus cuniculus | |
| 4-hydroxyphenylpyruvate | - |
Oryctolagus cuniculus | |
| acetoacetyl-CoA | - |
Bos taurus | |
| acetoacetyl-CoA | - |
Oryctolagus cuniculus | |
| acetoacetyl-CoA | - |
Rattus norvegicus | |
| ADP | - |
Bos taurus | |
| ADP | competitive | Oryctolagus cuniculus | |
| ADP | - |
Rattus norvegicus | |
| Ca2+ | weak | Oryctolagus cuniculus | |
| Clofibric acid | - |
Oryctolagus cuniculus | |
| Dichloroacetate | - |
Oryctolagus cuniculus | |
| Dichloroacetate | - |
Rattus norvegicus | |
| heparin | - |
Oryctolagus cuniculus | |
| isobutyryl-CoA | - |
Oryctolagus cuniculus | |
| isovaleryl-CoA | - |
Oryctolagus cuniculus | |
| malonyl-CoA | - |
Oryctolagus cuniculus | |
| methylmalonyl-CoA | - |
Bos taurus | |
| methylmalonyl-CoA | - |
Oryctolagus cuniculus | |
| methylmalonyl-CoA | - |
Rattus norvegicus | |
| Mg2+ | at concentrations above 1.5 mM, activation below | Oryctolagus cuniculus | |
| n-Octanoate | - |
Bos taurus | |
| n-Octanoate | - |
Oryctolagus cuniculus | |
| n-Octanoate | - |
Rattus norvegicus | |
| NADP+ | - |
Oryctolagus cuniculus | |
| phenylacetate | - |
Oryctolagus cuniculus | |
| Phenyllactate | - |
Oryctolagus cuniculus | |
| pyruvate | - |
Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
ATP | pH 7.35, 20°C | Oryctolagus cuniculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | part of intramitochondrial branched-chain 2-oxoacid dehydrogenase complex | Rattus norvegicus | 5739 | - |
| mitochondrion | part of intramitochondrial branched-chain 2-oxoacid dehydrogenase complex | Bos taurus | 5739 | - |
| mitochondrion | part of intramitochondrial branched-chain 2-oxoacid dehydrogenase complex | Oryctolagus cuniculus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| EGTA | activation, presumably by chelation of Ca2+ | Oryctolagus cuniculus | |
| Mg2+ | - |
Rattus norvegicus | |
| Mg2+ | - |
Bos taurus | |
| Mg2+ | maximum activity at 1.5 mM, inhibits above 1.5 mM | Oryctolagus cuniculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 2000000 | - |
above 2000000, gel filtration | Oryctolagus cuniculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] | Rattus norvegicus | phosphorylation inactivates EC 1.2.4.4 | ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate | - |
? | |
| ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] | Bos taurus | phosphorylation inactivates EC 1.2.4.4 | ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate | - |
? | |
| ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] | Oryctolagus cuniculus | phosphorylation inactivates EC 1.2.4.4 | ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Oryctolagus cuniculus | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
| alpha-ketoacid dehydrogenase complex | Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Oryctolagus cuniculus | - |
| heart | - |
Rattus norvegicus | - |
| kidney | - |
Rattus norvegicus | - |
| kidney | - |
Bos taurus | - |
| kidney | cortex | Oryctolagus cuniculus | - |
| liver | - |
Rattus norvegicus | - |
| liver | - |
Bos taurus | - |
| liver | - |
Oryctolagus cuniculus | - |
| skeletal muscle | - |
Rattus norvegicus | - |
| skeletal muscle | - |
Oryctolagus cuniculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] | 2 Ser-residues in E1-alpha-subunit | Rattus norvegicus | ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate | - |
? | |
| ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] | 2 Ser-residues in E1-alpha-subunit | Bos taurus | ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate | - |
? | |
| ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] | 2 Ser-residues in E1-alpha-subunit | Oryctolagus cuniculus | ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate | - |
? | |
| ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] | incorporates 0.75 mol phosphate per mol phosphorylation site and 1.5 mol/mol alpha-subunit | Oryctolagus cuniculus | ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate | - |
? | |
| ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] | phosphorylation inactivates EC 1.2.4.4 | Rattus norvegicus | ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate | - |
? | |
| ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] | phosphorylation inactivates EC 1.2.4.4 | Bos taurus | ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate | - |
? | |
| ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] | phosphorylation inactivates EC 1.2.4.4 | Oryctolagus cuniculus | ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
in decreasing order of activity: HEPES, potassium phosphate, imidazole, 3-(N-morpholino)ethane buffer | Oryctolagus cuniculus |
| 7.5 | - |
- |
Oryctolagus cuniculus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
ADP | pH 7.35, 20°C | Oryctolagus cuniculus | |
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