Activating Compound | Comment | Organism | Structure |
---|---|---|---|
O2 | hypoxia leads to time-dependent AMPK activation in ATII cells. Maximal activation of AMPK after 10 min of 1.5% O2 exposure, whereas 3% O2 activates AMPK in a similar but slower manner. AMPK levels return to the baseline after 30 min of hypoxia exposure. Hypoxia-generated mitochondrial reactive oxygen species leads to the activation of the AMPK alpha1 isoform at Thr172. Hypoxia fails to activate AMPK in mitochondrion-deficient rho0-A549 cells | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
ATII cells infected with a hemagglutinin-tagged adenovirus carrying the dominant-negative mutant K45R of the AMPK alpha1 subunit or with an adenovirus expressing a constitutively active AMPK alpha variant or with an adenovirus expressing a constitutively active AMPK alpha variant in which Thr172 is replaced with aspartate in the truncated AMPK alpha subunit, comprising residues 1 to 312. A549 cells transfected with AMPK alpha1 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K45R | dominant-negative, kinase-dead mutant of the AMPK alpha1 subunit | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-[4-(2-piperidin-1-yl-ethoxy)-phenyl]-3-pyridin-4-yl-pyrrazolo[1,5-a]-pyrimidine | compound C, hypoxia-induced PKCzeta translocation to the plasma membrane and phosphorylation at Thr410 is prevented by pharmacological inhibition of AMPK | Homo sapiens | |
additional information | overexpression of reactive oxygen species scavenger catalase prevents hypoxia-induced AMPK activation | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
alveolar epithelium | - |
Homo sapiens | - |
additional information | ATII cell | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetyl-CoA carboxylase | AMPK alpha phosphorylates at Ser79 | Homo sapiens | ADP + [acetyl-CoA carboxylase]phosphate | - |
? | |
ATP + PKZeta | AMPK alpha phosphorylates PKZeta on residue Thr410 within the PKCzeta activation loop | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AMP-activated protein kinase | - |
Homo sapiens |
AMPK | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | activation of AMPK alpha is necessary for hypoxia-induced AMPK-PKCzeta binding in alveolar epithelial cells. Overexpression of a dominant-negative AMPK alpha subunit construct prevents hypoxia-induced endocytosis of Na,K-ATPase, hypoxia-induced PKCzeta translocation to the plasma membrane and phosphorylation at Thr410 | Homo sapiens |