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Literature summary for 2.7.11.31 extracted from

  • Qin, S.; De Vries, G.W.
    alpha2 But not alpha1 AMP-activated protein kinase mediates oxidative stress-induced inhibition of retinal pigment epithelium cell phagocytosis of photoreceptor outer segments (2008), J. Biol. Chem., 283, 6744-6751.
    View publication on PubMed
Search for more literature for 2.7.11.31

Activating Compound

Activating Compound Comment Organism Structure
5-aminoimidazole-4-carboxamide riboside AICAR, its activation of AMPK is abolished by preincubation with dipyridamole or 5-iodotubercidin Homo sapiens
hydrogen peroxide sublethal oxidative stress inhibits retinal pigment epithelium cell phagocytosis and activates AMPK. 0.5 mM hydrogen peroxide dramatically activates AMPKalpha, reaches the peak within 15 min, and declines 1 h later. Thr172 phosphorylation of catalytic subunit AMPKalpha is required for AMPKalpha activation Homo sapiens

Application

Application Comment Organism
drug development activating AMPK may have therapeutic potential in treating dry macular degeneration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
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-
-

Source Tissue

Source Tissue Comment Organism Textmining
ARPE-19 cell
-
 Homo sapiens
-
retinal pigment epithelium alpha1 AMPK is the dominant isoform Homo sapiens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetyl-CoA carboxylase AMPKalpha can phosphorylate Ser79 of acetyl-CoA carboxylase Homo sapiens ADP + [acetyl-CoA carboxylase]phosphate
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 ?

Synonyms

Synonyms Comment Organism
AMP-activated protein kinase
-
 Homo sapiens
AMPK
-
 Homo sapiens

Expression

Organism Comment Expression
Homo sapiens alpha1 and alpha2 AMPK siRNAs selectively suppress AMPKalpha1 and AMPKalpha2 protein, respectively. AMPKalpha1 and AMPKalpha2 proteins are reduced to 6 and 1% of respective controls. Combined treatment with both alpha1 and alpha2 AMPK siRNAs lead to 66 and 92% reduction of AMPKalpha1 and AMPKalpha2 protein, respectively down
Homo sapiens AMPKalpha1 repression causes a 37% compensational increase in AMPKalpha2 protein up

General Information

General Information Comment Organism
physiological function AMPK activation during oxidative stress may switch retinal pigment epithelium cells to a self-protected status. Alpha2 but not alpha1 AMPK is involved in retinal pigment epithelium cell phagocytosis and activation of alpha2 AMPK contributes to the inhibition of retinal pigment epithelium cell phagocytosis by oxidative stress Homo sapiens