Activating Compound | Comment | Organism | Structure |
---|---|---|---|
(+)-simvastatin | 0.05 mM increases phosphorylation of AMPK at Thr172 by 2.6fold and acetyl-CoA carboxylase at Ser79 in BAEC. Ser428 phosphorylation of LKB1 is essential for statin-induced AMPK activation. Statin-induced AMPK activation in BAEC is independent of CaMKKbeta. Activation of AMPK by statin Is O2- or ONOO- dependent | Bos taurus | |
(+)-simvastatin | in vivo administration of statin increases 3-nitrotyrosine and the phosphorylation of AMPK and acetyl-CoA carboxylase in wild-type mice but not in mice deficient in endothelial nitric-oxide synthase. PKC-zeta-dependent AMPK activation. In vivo transfection of PKC-zeta-specific small interfering RNA in mice significantly attenuates statin-enhanced phosphorylation of AMPK-Thr172, acetyl-CoA carboxylase-Ser79, and LKB1-Ser428 | Mus musculus | |
(+)-simvastatin | LKB1 is required for statin-dependent AMPK activation. Transfection of LKB1-expressing plasmid is required for statin-induced AMPK activation in A-549 and HeLa S3 cell lines deficient in endogenous LKB1 | Homo sapiens | |
A23187 | 0.01 mM significantly enhances the phosphorylation of AMPK-Thr172 in BAEC. Either STO-609 (0.001 mM) or BAPTA-AM (0.02 mM), significantly suppresses calcium inophore A23187-enhanced phosphorylation in BAEC | Bos taurus | |
metformin | significantly increases AMPK activity in the aortas and hearts of wild-type mice but not those of eNOS-/-, although eNOS-/- mice express AMPK | Mus musculus | |
Mito-TEMPOL | mitochondria-targeting superoxide dismutase mimetic, 0.01 mM markedly attenuates statin-enhanced phosphorylation of both AMPK-Thr172 and acetyl-CoA carboxylase-Ser79 | Bos taurus | |
additional information | ONOO- dependent activation of AMPK | Mus musculus | |
PKC-zeta | is required for statin-induced LKB1 nucleus export and AMPK activation in HUVEC cells | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
BAEC infected with adenovirus expressing an AMPK dominant negative mutant | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-[4-(2-piperidin-1-yl-ethoxy)-phenyl]-3-pyridin-4-yl-pyrrazolo[1,5-a]-pyrimidine | compound C, abolishes statin-induced reduction of O2- in BAEC | Bos taurus | |
additional information | genetic inhibition of LKB1 ablates statin-induced AMPK activation in endothelial cells | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Homo sapiens | - |
- |
- |
Mus musculus | - |
C57BL/6J mice | - |
Mus musculus C57/BL6J | - |
C57BL/6J mice | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A-549 cell | - |
Homo sapiens | - |
aorta | - |
Mus musculus | - |
aortic endothelial cell | - |
Bos taurus | - |
BAEC cell | - |
Bos taurus | - |
heart | - |
Mus musculus | - |
HeLa-S3 cell | - |
Homo sapiens | - |
HUVEC cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetyl-CoA carboxylase | - |
Mus musculus | ADP + phosphorylated acetyl-CoA carboxylase | - |
? | |
ATP + acetyl-CoA carboxylase | - |
Mus musculus C57/BL6J | ADP + phosphorylated acetyl-CoA carboxylase | - |
? | |
ATP + acetyl-CoA carboxylase | - |
Homo sapiens | ADP + [acetyl-CoA carboxylase]phosphate | - |
? | |
ATP + acetyl-CoA carboxylase | - |
Bos taurus | ADP + [acetyl-CoA carboxylase]phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AMP-activated protein kinase | - |
Mus musculus |
AMP-activated protein kinase | - |
Homo sapiens |
AMP-activated protein kinase | - |
Bos taurus |
AMPK | - |
Mus musculus |
AMPK | - |
Homo sapiens |
AMPK | - |
Bos taurus |
Organism | Comment | Expression |
---|---|---|
Bos taurus | overexpression of AMPK dominant negative mutant abolishes statin-induced reduction of O2- in BAEC | additional information |
General Information | Comment | Organism |
---|---|---|
physiological function | AMPK activation inhibits endothelial apoptosis in cultured cells. AMPK activation suppresses the basal and angiotensin II-enhanced superoxide anions in BAEC | Bos taurus |