Cloned (Comment) | Organism |
---|---|
transient transfection of GST-tagged enzyme into HEK-293 cells coexpression with recombinant tau, coexpression of wild-type GSK-3alpha and wild-type GSK-3beta enhances Tau phosphorylation at Ser396 and Ser404 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K148R | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type | Homo sapiens |
K85R | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type | Homo sapiens |
additional information | deletion of the C-terminus to result in mutants alphaDELTACT1-4, with remaining 448, 441, 430, and 416 residues of 483, respectively, leads to loss of nearly all activity of the GSK-3 isoform and abolishes suppression of Wnt signaling | Homo sapiens |
additional information | deletion of the C-terminus to result in mutants betaDELTACT1-4, with remaining 385, 378, 367, and 344 residues of 420, respectively, leads to loss of nearly all activity of the GSK-3 isoform and abolishes suppression of Wnt signaling | Homo sapiens |
P379A/P380A | site-directed mutagenesis, the mutant shows rediuced activity compared to the wild-type | Homo sapiens |
P442A/P443A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type | Homo sapiens |
R159A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type | Homo sapiens |
R96A | site-directed mutagenesis, the mutant activity is similar to the wild-type | Homo sapiens |
S9A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type | Homo sapiens |
T390A | site-directed mutagenesis, the mutant activity is similar to the wild-type | Homo sapiens |
Y216F | site-directed mutagenesis, the mutant activity is similar to the wild-type | Homo sapiens |
Y279F | site-directed mutagenesis, the mutant activity is similar to the wild-type | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [tau-protein] | Homo sapiens | - |
ADP + O-phospho-[tau-protein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P49840 | isozyme alpha | - |
Homo sapiens | P49841 | isozyme beta | - |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged enzyme from HEK-293 cells by glutathione affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [tau-protein] | - |
Homo sapiens | ADP + O-phospho-[tau-protein] | - |
? | |
additional information | GSK-3alpha-specific requirement for priming of tau protein | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glycogen synthase kinase-3 | - |
Homo sapiens |
GSK-3 | - |
Homo sapiens |
GSK-3alpha | - |
Homo sapiens |
GSK-3beta | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | deletion of the C-terminus alphaDELTACT-4 leads to loss of nearly all activity of the GSK-3 isoform and abolishes suppression of Wnt signaling, GSK-3 C-terminal deletions mutants fail to interact with axin GID | Homo sapiens |
malfunction | deletion of the C-terminus betaDELTACT-4 leads to loss of nearly all activity of the GSK-3 isoform and abolishes suppression of Wnt signaling, GSK-3 C-terminal deletions mutants fail to interact with axin GID | Homo sapiens |
additional information | amino acids 345367 of GSK-3beta are essential for enzyme activity with tau protein, structure-function analysis of GSK-3alpha and GSK-3beta in mammalian cells | Homo sapiens |
additional information | amino acids 417-430 of GSK-3alpha are essential for enzyme activity with tau protein, structure-function analysis of GSK-3alpha and GSK-3beta in mammalian cells | Homo sapiens |
physiological function | glycogen synthase kinase-3 (GSK-3) isoforms, GSK-3alpha and GSK-3beta, are serine/threonine kinases involved in numerous cellular processes and diverse diseases, including Alzheimer disease, cancer, and diabetes. GSK-3 isoforms function redundantly in some settings, while, in others, they exhibit distinct activities. In the canonical Wnt signaling pathway, GSK-3 phosphorylation of beta-catenin is regulated via interactions with the scaffold protein axin | Homo sapiens |
physiological function | in the canonical Wnt signaling pathway, GSK-3 phosphorylation of beta-catenin is regulated via interactions with the scaffold protein axin. Glycogen synthase kinase-3 (GSK-3) isoforms, GSK-3alpha and GSK-3beta, are serine/threonine kinases involved in numerous cellular processes and diverse diseases, including Alzheimer disease, cancer, and diabetes. GSK-3 isoforms function redundantly in some settings, while, in others, they exhibit distinct activities. In the canonical Wnt signaling pathway, GSK-3 phosphorylation of beta-catenin is regulated via interactions with the scaffold protein axin | Homo sapiens |