Cloned (Comment) | Organism |
---|---|
TTBK2 kinase domain (residues 1-331) of TTBK2 subcloned into pBiEx-3 vector, expressed in insect cells with a baculovirus overexpression system | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
by hanging-drop vapour-diffusion method, crystals belong to space group P212121, with unit-cell parameters a = 55.6, b = 113.7, c = 117.3 A, alpha = beta = gamma = 90.0°, to 2.9 A resolution | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
gel filtration | Homo sapiens |
38620 | - |
calculated | Homo sapiens |
39000 | - |
1 * 39000, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
centrifugation, sonication, gel filtration, metal affinity chromatography, to homogeneity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + protein tau | TTBK2 (1-331) phosphorylates residues Ser208 and Ser210 | Homo sapiens | ADP + protein tau phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 39000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
tau-tubulin kinase 2 | - |
Homo sapiens |
TTBK2 | - |
Homo sapiens |