Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | MPK6 is activated by MKK4 MAPK kinase | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged MPK6 | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
U0126 | the activity of MAPKs is inhibited by treatment of cells with the selective MEK inhibitor | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microtubule | mitotic microtubules | Arabidopsis thaliana | 5874 | - |
microtubule | mitotic microtubules, MPK6 is recruited to gamma-tubulin or gamma-tubulin complexes | Arabidopsis thaliana | 5874 | - |
additional information | co-localization of MPK6 and p-ERK with gamma-tubulin and with microtubule plus end protein EB1c in Arabidopsis cells. gamma-Tubulin and ERK partly colocalize on the spindle and prominent close to kinetochores. p-ERK follows the dynamic localization of gamma-tubulin in the anaphase-to-telophase transition in Arabidopsis cells. In anaphase, phosphorylated ERK and EB1c-GFP co-localize on shortening kinetochore fibres | Arabidopsis thaliana | - |
- |
additional information | co-localization of MPK6 and phosphorylated ERK with gamma-tubulin and with microtubule plus end protein EB1c in Arabidopsis cells | Arabidopsis thaliana | - |
- |
nucleus | - |
Arabidopsis thaliana | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + EB1c | Arabidopsis thaliana | the microtubule plus end protein | ADP + phosphorylated EB1c | - |
? | |
additional information | Arabidopsis thaliana | MPK6 interacts with gamma-tubulin and co-sediments with plant microtubules polymerized in vitro. The active form of MAP kinase is enriched with microtubules and follows similar dynamics to gamma-tubulin, moving from poles to midzone during the anaphase-to-telophase transition | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
ecotypes Landsberg erecta (Ler) and Columbia (Col) | - |
Arabidopsis thaliana | Q39026 | ecotypes Landsberg erecta (Ler) and Columbia (Col) | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | MPK6 is activated by MKK4 MAPK kinase | Arabidopsis thaliana |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged MPK6 by metal affinity chromatography, the His-tag is cleaved off by TEV protease | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | ERK is associated with gamma-tubulin on microtubules in proliferating cells | Arabidopsis thaliana | - |
cell culture | MPK6 is associated with gamma-tubulin on microtubules in proliferating cells | Arabidopsis thaliana | - |
root meristematic cell | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + EB1c | the microtubule plus end protein | Arabidopsis thaliana | ADP + phosphorylated EB1c | - |
? | |
ATP + EB1c | the microtubule plus end protein, recombinant GST-tagged substrate protein, phosphorylation on a threonine residue | Arabidopsis thaliana | ADP + phosphorylated EB1c | - |
? | |
ATP + MAP65-1 | microtubule-associated protein, phosphorylation in vitro by MPK6, recombinant GST-tagged substrate protein | Arabidopsis thaliana | ADP + phosphorylated MAP65-1 | - |
? | |
additional information | MPK6 interacts with gamma-tubulin and co-sediments with plant microtubules polymerized in vitro. The active form of MAP kinase is enriched with microtubules and follows similar dynamics to gamma-tubulin, moving from poles to midzone during the anaphase-to-telophase transition | Arabidopsis thaliana | ? | - |
? | |
additional information | no activity with recombinant GST-tagged EB1a protein. MPK6 is recruited to gamma-tubulin or gamma-tubulin complexes, but no direct phosphorylation of either gamma-tubulin or gamma-tubulin complex protein GCP4 wby MPK6 is detectable in vitro | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ERK | - |
Arabidopsis thaliana |
MAPK6 | - |
Arabidopsis thaliana |
mitogen-activated protein kinase | - |
Arabidopsis thaliana |
mitogen-activated protein kinase 6 | - |
Arabidopsis thaliana |
MPK6 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
room temperature | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | the mpk6-2 mutant is sensitive to 3-nitro-L-tyrosine (NO2-Tyr) treatment with respect to mitotic abnormalities, and root cells overexpressing the MAP kinase-inactivating phosphatase AP2C3 show defects in chromosome segregation and spindle orientation | Arabidopsis thaliana |
physiological function | the active form of MAP kinase interacts with gamma-tubulin on specific subsets of mitotic microtubules during late mitosis. MPK6 phosphorylates EB1c, but not EB1a, and has a role in maintaining regular planes of cell division under stress conditions. MPK6 activtes the micotubule-associated protein MAP65-1 that has a redundant function in Arabidopsis with MAP65-3 in cytokinesis. MPK6 is required for regulation of the alignment of cell division on NO2-Tyr treatment | Arabidopsis thaliana |