Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | p38 MAPK is activated by phosphorylation. Inhibition of SCD-1 decreases the proportion of monounsaturated fatty acid-containing phospholipids and activates p38 MAPK | Mus musculus | |
palmitate | activates p38 MAPK phosphorylation and activates it | Mus musculus |
Cloned (Comment) | Organism |
---|---|
quantitative RT-PCR enzyme expression analysis | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme silencing by selective siRNA | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | some monounsaturated fatty acids inhibit p38 MAPK via selective protein-lipid interactions | Mus musculus | |
skepinone-L | the specificity by which SCD-1 modulates the phospholipid composition and inhibits p38 MAPK signaling (among survival/stress pathways), thereby preventing endoplasmic reticulum stress (but not other SCD-1-dependent responses), suggests selective protein-lipid interactions | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Mus musculus | 5783 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | p38 MAPK is activated by phosphorylation | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
3T3-L1 cell | - |
Mus musculus | - |
fibroblast | - |
Mus musculus | - |
NIH-3T3 cell | - |
Mus musculus | - |
Synonyms | Comment | Organism |
---|---|---|
p38 MAPK | - |
Mus musculus |
p38 mitogen-activated protein kinase | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mus musculus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000025 | 0.00005 | pH 7.4, 22°C | Mus musculus | skepinone-L |
General Information | Comment | Organism |
---|---|---|
metabolism | endoplasmic reticulum homeostasis is regulated by a network of signaling pathways which include stearoyl-CoA desaturase (SCD)-1, p38 mitogen-activated protein kinase (MAPK) and the unfolded protein response (UPR). All these pathways are located at the interface of cell cycle control and cell stress. Inhibition or silencing of SCD-1, via inhibitor CAY10566 or siRNA, specifically induces phosphorylation and activation of p38 MAPK. SCD-1 counteracts palmitate-induced endoplasmic reticulum stress by reducing p38 MAPK activation. Role of SCD-1 and p38 MAPK for neutral lipid biosynthesis, cell proliferation, and viability and insulin-dependent glucose uptake, overview | Mus musculus |
additional information | p38 MAPK might show selective protein-lipid interactions | Mus musculus |
physiological function | role of p38 mitogen-activated protein kinase in linking stearoyl-CoA desaturase-1 activity with endoplasmic reticulum homeostasis. During lipotoxic and cell cycle stress, prolonged activation of p38 MAPK due to SCD-1 inhibition induced endoplasmic reticulum stress, the unfolded protein response, and endoplasmic reticulum/Golgi remodeling. The negative regulation of p38 MAPK mediates the protective effects of SCD-1 on endoplasmic reticulum homeostasis under distinct stress conditions. Role of SCD-1 and p38 MAPK for neutral lipid biosynthesis, cell proliferation, and viability and insulin-dependent glucose uptake, overview | Mus musculus |