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Literature summary for 2.7.11.24 extracted from

  • Leissing, F.; Nomoto, M.; Bocola, M.; Schwaneberg, U.; Tada, Y.; Conrath, U.; Beckers, G.J.
    Substrate thiophosphorylation by Arabidopsis mitogen-activated protein kinases (2016), BMC Plant Biol., 16, 48.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.24

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme is phosphorylated and activated by a mitogen-activated protein kinase kinase, GST-tagged MPK3 is activated by constitutively active MKK4 and MKK5, which cause a dual phosphorylation of the TEY motif in the activation loop Arabidopsis thaliana
additional information the enzyme is phosphorylated and activated by a mitogen-activated protein kinase kinase, GST-tagged MPK4 is activated by constitutively active MKK1 and MKK2, which cause a dual phosphorylation of the TEY motif in the activation loop Arabidopsis thaliana
additional information the enzyme is phosphorylated and activated by a mitogen-activated protein kinase kinase, GST-tagged MPK6 is activated by constitutively active MKK4 and MKK5, which cause a dual phosphorylation of the TEY motif in the activation loop Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of GST-tagged MPK3 in Escherichia coli Arabidopsis thaliana
recombinant expression of GST-tagged MPK4 in Escherichia coli Arabidopsis thaliana
recombinant expression of GST-tagged MPK6 in Escherichia coli Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + Arabidopsis thaliana protein AT1G7815 Arabidopsis thaliana
-
 ADP + phosphorylated Arabidopsis thaliana proteins AT1G78150
-
 ?
ATP + Arabidopsis thaliana protein AT2G26530 Arabidopsis thaliana
-
 ADP + phosphorylated Arabidopsis thaliana proteins AT2G26530
-
 ?
ATP + Arabidopsis thaliana protein AT3G11330 Arabidopsis thaliana
-
 ADP + phosphorylated Arabidopsis thaliana proteins AT3G11330
-
 ?
ATP + Arabidopsis thaliana protein AT4G38710 Arabidopsis thaliana
-
 ADP + phosphorylated Arabidopsis thaliana proteins AT4G38710
-
 ?
ATP + myelin basic protein Arabidopsis thaliana
-
 ADP + phosphorylated myelin basic protein
-
 ?
ATPgammaS + myelin basic protein Arabidopsis thaliana Arabidopsis thaliana MPKs use ATPgammaS to thiophosphorylate myelin basic protein ADP + thiophosphorylated myelin basic protein
-
 ?
additional information Arabidopsis thaliana FLAG-tagged Arabidopsis thaliana proteins AT1G78150, AT2G26530, AT3G11330, and AT4G38710 are good MPK3/6 substrates, but are poor substrates for the closely related Arabidopsis thaliana MPK4 ?
-
 ?
N6-benzyl-ATPgammaS + myelin basic protein Arabidopsis thaliana Arabidopsis thaliana MPK3 mutant T119A uses N6-benzyl-ATPgammaS to thiophosphorylate myelin basic protein ADP + benzylthiophosphorylated myelin basic protein
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q39023
-
-
Arabidopsis thaliana Q39024
-
-
Arabidopsis thaliana Q39026
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein the enzyme is phosphorylated and activated by a mitogen-activated protein kinase kinase, GST-tagged MPK3 is activated by constitutively active MKK4 and MKK5, which cause a dual phosphorylation of the TEY motif in the activation loop Arabidopsis thaliana
phosphoprotein the enzyme is phosphorylated and activated by a mitogen-activated protein kinase kinase, GST-tagged MPK4 is activated by constitutively active MKK1 and MKK2, which cause a dual phosphorylation of the TEY motif in the activation loop Arabidopsis thaliana
phosphoprotein the enzyme is phosphorylated and activated by a mitogen-activated protein kinase kinase, GST-tagged MPK6 is activated by constitutively active MKK4 and MKK5, which cause a dual phosphorylation of the TEY motif in the activation loop Arabidopsis thaliana

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged MPK3 by affinity chromatography from Escherichia coli Arabidopsis thaliana
recombinant GST-tagged MPK4 by affinity chromatography from Escherichia coli Arabidopsis thaliana
recombinant GST-tagged MPK6 by affinity chromatography from Escherichia coli Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + Arabidopsis thaliana protein AT1G7815
-
 Arabidopsis thaliana ADP + phosphorylated Arabidopsis thaliana proteins AT1G78150
-
 ?
ATP + Arabidopsis thaliana protein AT2G26530
-
 Arabidopsis thaliana ADP + phosphorylated Arabidopsis thaliana proteins AT2G26530
-
 ?
ATP + Arabidopsis thaliana protein AT3G11330
-
 Arabidopsis thaliana ADP + phosphorylated Arabidopsis thaliana proteins AT3G11330
-
 ?
ATP + Arabidopsis thaliana protein AT4G38710
-
 Arabidopsis thaliana ADP + phosphorylated Arabidopsis thaliana proteins AT4G38710
-
 ?
ATP + myelin basic protein
-
 Arabidopsis thaliana ADP + phosphorylated myelin basic protein
-
 ?
ATPgammaS + myelin basic protein Arabidopsis thaliana MPKs use ATPgammaS to thiophosphorylate myelin basic protein Arabidopsis thaliana ADP + thiophosphorylated myelin basic protein
-
 ?
additional information FLAG-tagged Arabidopsis thaliana proteins AT1G78150, AT2G26530, AT3G11330, and AT4G38710 are good MPK3/6 substrates, but are poor substrates for the closely related Arabidopsis thaliana MPK4 Arabidopsis thaliana ?
-
 ?
N6-benzyl-ATPgammaS + myelin basic protein Arabidopsis thaliana MPK3 mutant T119A uses N6-benzyl-ATPgammaS to thiophosphorylate myelin basic protein Arabidopsis thaliana ADP + benzylthiophosphorylated myelin basic protein
-
 ?

Subunits

Subunits Comment Organism
More residue Y119 is the gatekeeper in MPK3, three-dimensional structure analysis Arabidopsis thaliana
More residue Y124 is the gatekeeper in MPK4, three-dimensional structure analysis Arabidopsis thaliana
More residue Y144 is the gatekeeper in MPK6, three-dimensional structure analysis Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
MPK3
-
 Arabidopsis thaliana
MPK4
-
 Arabidopsis thaliana
MPK6
-
 Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Arabidopsis thaliana
ATPgammaS MPK3 accepts ATPgammaS as a phosphoryldonor to thiophosphorylate myelin basic protein and supposedly also other substrate proteins Arabidopsis thaliana
ATPgammaS MPK4 accepts ATPgammaS as a phosphoryldonor to thiophosphorylate myelin basic protein and supposedly also other substrate proteins Arabidopsis thaliana
ATPgammaS MPK6 accepts ATPgammaS as a phosphoryldonor to thiophosphorylate myelin basic protein and supposedly also other substrate proteins Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction MKKs 1 and 2 induce dual phosphorylation of the TEY motif in the activation loop of the kinase-death version of MPK4KD, but do not stimulate MPK4KD to phosphorylate myelin basic protein Arabidopsis thaliana
malfunction MKKs 4 and 5 induce dual phosphorylation of the TEY motif in the activation loop of the kinase-death version of MPK3KD, but do not stimulate MPK3KD to phosphorylate myelin basic protein Arabidopsis thaliana
malfunction MKKs 4 and 5 induce dual phosphorylation of the TEY motif in the activation loop of the kinase-death version of MPK6KD, but do not stimulate MPK6KD to phosphorylate myelin basic protein Arabidopsis thaliana
additional information residue Y119 is the gatekeeper in the MPK3 enzyme structure Arabidopsis thaliana
additional information residue Y124 is the gatekeeper in the MPK4 enzyme structure Arabidopsis thaliana
additional information residue Y144 is the gatekeeper in the MPK6 enzyme structure Arabidopsis thaliana