Literature summary for 2.7.11.24 extracted from

Fernandes, N.; Bailey, D.E.; Vanvranken, D.L.; Allbritton, N.L.
Use of docking peptides to design modular substrates with high efficiency for mitogen-activated protein kinase extracellular signal-regulated kinase (2007), ACS Chem. Biol., 2, 665-673.

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Cloned(Commentary)

Cloned (Comment)	Organism
His6-tagged ERK1 and p38alpha kinase expressed in Escherichia coli strain BL21 (DE3)	synthetic construct

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0006	-	STE7ERK	ERK1	synthetic construct
0.0037	-	MEK1ERK	ERK1	synthetic construct
0.0044	-	ELKERK	ERK1	synthetic construct
0.0056	-	MEK2ERK	ERK1	synthetic construct
0.03	-	MEK2ERK	p38alpha kinase	synthetic construct
0.065	-	MEK1ERK	p38alpha kinase	synthetic construct
0.096	-	SCRAMMMEK2	ERK1	synthetic construct
0.127	-	ERKSub	ERK1	synthetic construct
0.173	-	ERKSTE7	ERK1	synthetic construct
0.344	-	ERKMEK1	ERK1	synthetic construct
0.388	-	ERKMEK2	ERK1	synthetic construct
1.2	-	ERKSub	p38alpha kinase	synthetic construct

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	synthetic construct

Organism

Organism	UniProt	Comment	Textmining
synthetic construct	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ERK1 and p38alpha kinase purified by NiNTA column	synthetic construct

Storage Stability

Storage Stability	Organism
-70°C, 12.5 mM HEPES-buffer, pH 7.3, 100 mM KCl, 6.25% (v/v) glycerol, 0.5 mM DTT	synthetic construct

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + ELKERK	ERK1	synthetic construct	?	-	?
ATP + ERKMEK1	ERK1	synthetic construct	?	-	?
ATP + ERKMEK2	ERK1	synthetic construct	?	-	?
ATP + ERKSTE7	ERK1	synthetic construct	?	-	?
ATP + ERKSub	ERK1 and p38alpha kinase	synthetic construct	?	-	?
ATP + MEK1ERK	ERK1 and p38alpha kinase	synthetic construct	?	-	?
ATP + MEK2ERK	ERK1 and p38alpha kinase	synthetic construct	?	-	?
ATP + SCRAMMMEK2	ERK1	synthetic construct	?	-	?
ATP + STE7ERK	ERK1	synthetic construct	?	-	?
additional information	a peptide docking sequence derived from either a downstream substrate or an upstream activator is appended to an ERK substrate peptide to yield a high-efficiency substrate for ERK without loss of specificity	synthetic construct	?	-	?

Synonyms

Synonyms	Comment	Organism
ERK1	-	synthetic construct
p38alpha kinase	-	synthetic construct

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.97	-	MEK2ERK	p38alpha kinase	synthetic construct
2	-	MEK1ERK	-	synthetic construct
2.167	-	SCRAMMMEK2	-	synthetic construct
2.3	-	MEK2ERK	-	synthetic construct
2.3	-	ELKERK	-	synthetic construct
2.67	-	ERKSTE7	-	synthetic construct
2.83	-	ERKMEK1	-	synthetic construct
2.83	-	STE7ERK	-	synthetic construct
2.94	-	SCRAMMMEK2	-	synthetic construct
3	-	ERKMEK2	-	synthetic construct
3.13	-	ERKSub	-	synthetic construct
4.167	-	ERKSub	-	synthetic construct
9.5	-	ERKSub	p38alpha kinase	synthetic construct
9.5	-	MEK1ERK	p38alpha kinase	synthetic construct
16.67	-	MEK2ERK	p38alpha kinase	synthetic construct

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Release 2023.1 (January 2023)