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Literature summary for 2.7.11.24 extracted from

  • Szafranska, A.E.; Luo, X.; Dalby, K.N.
    Following in vitro activation of mitogen-activated protein kinases by mass spectrometry and tryptic peptide analysis: purifying fully activated p38 mitogen-activated protein kinase alpha (2005), Anal. Biochem., 336, 1-10.
    View publication on PubMed
Search for more literature for 2.7.11.24

Activating Compound

Activating Compound Comment Organism Structure
additional information the recombinant detagged enzyme is activated by specific phosphorylation at Thr180 and Tyr182 through recombinant GST-tagged MKK6 mutant S207E/T211E Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged p38 isozyme alpha in Escherichia coli strain BL21(DE3) Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0019
-
 protein ATF2 pH 7.6, 27°C, purified, recombinant detagged, activated p38 MAPKalpha Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ as MgATP2- Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the p38 MAPKalpha is involved in cell signal transduction and mediates responses to cell stresses and to growth factors ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein p38 MAPKalpha is activated by phosphorylation at Thr180 and Tyr182 through recombinant GST-tagged MKK6 mutant S207E/T211E, for activation and specific dual phosphorylation of the recombinant enzyme, the His-tag needs to be removed by thrombin, because it can cause unspecific phosphorylation at other sites Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged p38 isozyme alpha from Escherichia coli strain BL21(DE3) by nickel affinity and ion exchange chromatography to over 98% purity, removal of the His-tag by thrombin is required for propper enzyme purification and activation Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + protein ATF2 recombinant GST-tagged ATF2DELTA115 Homo sapiens ADP + phosphorylated protein ATF2
-
 ?
additional information the p38 MAPKalpha is involved in cell signal transduction and mediates responses to cell stresses and to growth factors Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 43472-43556, unphosphorylated to trifold phosphorylated p38 MAPKalpha, mass spectrometry Homo sapiens

Synonyms

Synonyms Comment Organism
p38 MAPKalpha
-
 Homo sapiens
p38 mitogen-activated protein kinase alpha
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
27
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.98
-
 protein ATF2 pH 7.6, 27°C, purified, recombinant detagged, activated p38 MAPKalpha Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP as MgATP2- Homo sapiens