Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | structural basis for the recognition of calmodulin by eukaryotic elongation factor 2 kinase, NMR spectrometric analysis, overview. The enzyme binds Ca2+-loaded CaM with high affinity largely through the CaM C lobe, but C-lobe-mediated interactions occur in a Ca2+-independent fashion. the lobe lacks bound calcium ions even under high calcium conditions. Conserved eEF-2K residue W85 anchors it to CaM by inserting into a deep hydrophobic cavity within the CaM C lobe. Mutation of residue W85 to S85 substantially weakens interactions between full-length eEF-2K and CaM in vitro and reduces eEF-2 phosphorylation in cells | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
recombinant coexpression of full-length human calmodulin (CaM), the predicted calmodulin-binding segment of human eEF-2K, residues 74-100 (eEF-2KCBD), and full-length eEF-2K | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
W85S | site-directed mutagenesis, Mutation of residue W85 to S85 substantially weakens interactions between full-length eEF-2K and CaM in vitro and reduces eEF-2 phosphorylation in cells | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Homo sapiens | |
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [elongation factor 2] | Homo sapiens | - |
ADP + [elongation factor 2] phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O00418 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant full-length human calmodulin (CaM), the predicted calmodulin-binding segment of human eEF-2K, residues 74-100 (eEF-2KCBD), and full-length eEF-2K | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [elongation factor 2] | - |
Homo sapiens | ADP + [elongation factor 2] phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EEF-2K | - |
Homo sapiens |
eukaryotic elongation factor 2 kinase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | eukaryotic elongation factor 2 kinase is a member of the atypical alpha-kinase family | Homo sapiens |
malfunction | mutation of the hydrophobic CaM anchor on eEF-2K disrupts its cellular activity. Mutation of residue W85 to S85 substantially weakens interactions between full-length eEF-2K and CaM in vitro and reduces eEF-2 phosphorylation in cells | Homo sapiens |
additional information | solution structure of the Ca2+-CaM-eEF-2KCBD complex, structure analysis, NMR spectrometric analysis, detailed overview | Homo sapiens |
physiological function | eukaryotic elongation factor 2 kinase (eEF-2K) phosphorylates eEF-2 leading to a decrease in global protein synthesis | Homo sapiens |