Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | required | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Mus musculus | |
Mg2+ | required | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [elongation factor 2] | Mus musculus | - |
ADP + [elongation factor 2] phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | O08796 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hematopoietic stem cell | - |
Mus musculus | - |
ovary | - |
Mus musculus | - |
skeletal muscle | - |
Mus musculus | - |
small intestine | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [elongation factor 2] | - |
Mus musculus | ADP + [elongation factor 2] phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
eEF2K | - |
Mus musculus |
elongation factor-2 kinase | - |
Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | knockout of eEF2K protects mice from a lethal dose of whole-body ionizing radiation at 8 Gy by reducing apoptosis levels in both bone marrow and gastrointestinal tracts. eEF2K deficiency results in more severe damage to the gastrointestinal tract at 20 Gy with the increased mitotic cell death in small intestinal stem cells. Elevation of Akt/ERK activity as well as the reduction of p21 expression occur in Eef2k-/- cells Eef2k-/- mice display opposite sensitivities at low dose and high dose of ionizing radiation according to tissue and cell types, overview. Consistent with the role of eEF2K in apoptosis, cleaved caspase-3-positive and TUNEL-positive cells are reduced in Eef2k-deficient mice | Mus musculus |
physiological function | elongation factor-2 kinase (eEF2K), a protein kinase that suppresses protein synthesis during elongation phase, is a positive regulator of apoptosis both in vivo and in vitro. eEF2K is required for G2/M arrest induced by radiation to prevent mitotic catastrophe in a p53-independent manner in epithelial cells. eEF2K also provides a protective strategy to maintain genomic integrity by arresting cell cycle in response to stress. Protective versus pro-apoptotic roles of eEF2K depend on the type of cells: eEF2K is protective in highly proliferative cells, such as small intestinal stem cells and cancer cells, which are more susceptible to mitotic catastrophe. The dynamics of eEF2K activity in DNA damage response is distinct in different cell types and are related to cell proliferating status. eEF2K suppresses intestinal mitotic cell death in response to gamma-irradiation | Mus musculus |