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Literature summary for 2.7.11.20 extracted from

  • Liao, Y.; Chu, H.P.; Hu, Z.; Merkin, J.J.; Chen, J.; Liu, Z.; Degenhardt, K.; White, E.; Ryazanov, A.G.
    Paradoxical roles of elongation factor-2 kinase in stem cell survival (2016), J. Biol. Chem., 291, 19545-19557.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
Calmodulin required Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Mus musculus
Mg2+ required Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [elongation factor 2] Mus musculus
-
ADP + [elongation factor 2] phosphate
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus O08796
-
-

Source Tissue

Source Tissue Comment Organism Textmining
hematopoietic stem cell
-
Mus musculus
-
ovary
-
Mus musculus
-
skeletal muscle
-
Mus musculus
-
small intestine
-
Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [elongation factor 2]
-
Mus musculus ADP + [elongation factor 2] phosphate
-
?

Synonyms

Synonyms Comment Organism
eEF2K
-
Mus musculus
elongation factor-2 kinase
-
Mus musculus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Mus musculus

General Information

General Information Comment Organism
malfunction knockout of eEF2K protects mice from a lethal dose of whole-body ionizing radiation at 8 Gy by reducing apoptosis levels in both bone marrow and gastrointestinal tracts. eEF2K deficiency results in more severe damage to the gastrointestinal tract at 20 Gy with the increased mitotic cell death in small intestinal stem cells. Elevation of Akt/ERK activity as well as the reduction of p21 expression occur in Eef2k-/- cells Eef2k-/- mice display opposite sensitivities at low dose and high dose of ionizing radiation according to tissue and cell types, overview. Consistent with the role of eEF2K in apoptosis, cleaved caspase-3-positive and TUNEL-positive cells are reduced in Eef2k-deficient mice Mus musculus
physiological function elongation factor-2 kinase (eEF2K), a protein kinase that suppresses protein synthesis during elongation phase, is a positive regulator of apoptosis both in vivo and in vitro. eEF2K is required for G2/M arrest induced by radiation to prevent mitotic catastrophe in a p53-independent manner in epithelial cells. eEF2K also provides a protective strategy to maintain genomic integrity by arresting cell cycle in response to stress. Protective versus pro-apoptotic roles of eEF2K depend on the type of cells: eEF2K is protective in highly proliferative cells, such as small intestinal stem cells and cancer cells, which are more susceptible to mitotic catastrophe. The dynamics of eEF2K activity in DNA damage response is distinct in different cell types and are related to cell proliferating status. eEF2K suppresses intestinal mitotic cell death in response to gamma-irradiation Mus musculus