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Literature summary for 2.7.11.20 extracted from
- Determinants for substrate phosphorylation by Dictyostelium myosin II heavy chain kinases A and B and eukaryotic elongation factor-2 kinase (2008), Biochim. Biophys. Acta, 1784, 908-915.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Calmodulin | required | Mus musculus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| enzyme expression as GST-tagged protein in Escherichia coli strain BL21(DE3) | Mus musculus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required | Mus musculus | |
| Mg2+ | required | Mus musculus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + [eukaryotic translation elongation factor 2] | Mus musculus | eEF2 is involved in eEF2 in neurite outgrowth regulation mechanism, since phosphorylated eEF2 inhibits mRNA translation via inhibition of eEF2-ribosome binding, calcium elevation appears to inhibit mRNA translation in growth cones by a synergistic mechanism involving regulation of EF2K, S6K, and eEF2 itself, overview | ADP + [eukaryotic translation elongation factor 2] phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | phosphorylation by p70-S6 kinase inhibits EF2K, Ca2+ decreases the phosphorylation of the enzyme | Mus musculus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GST-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + [eukaryotic translation elongation factor 2] | eEF2 is involved in eEF2 in neurite outgrowth regulation mechanism, since phosphorylated eEF2 inhibits mRNA translation via inhibition of eEF2-ribosome binding, calcium elevation appears to inhibit mRNA translation in growth cones by a synergistic mechanism involving regulation of EF2K, S6K, and eEF2 itself, overview | Mus musculus | ADP + [eukaryotic translation elongation factor 2] phosphate | - |
? | |
| ATP + [eukaryotic translation elongation factor 2] | the enzyme exhibits strong preference for threonine as the phosphoacceptor amino acid, substrate specificity and phosphoacceptor amino acid specificity, overview | Mus musculus | ADP + [eukaryotic translation elongation factor 2] phosphate | - |
? | |
| ATP + [MHCK A peptide substrate] | i.e. YAYDTRYRR | Mus musculus | ADP + [MHCK A peptide substrate] phosphate | - |
? | |
| additional information | a lysine or arginine in the P+1 position on the C-terminal side of the phosphoacceptor threonine, P site, is critical for peptide substrate recognition by eEF-2K, eEF-2K requires basic residues in both the P+1 and P+3 positions to recognize peptide substrates, overview | Mus musculus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EEF-2K | - |
Mus musculus |
| eukaryotic elongation factor-2 kinase | - |
Mus musculus |
| More | MHCKs and eEF-2Ks comprise a distinct subgroup within the alpha kinase famiily | Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Mus musculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Mus musculus | |
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Release 2023.1 (January 2023)
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