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Literature summary for 2.7.11.2 extracted from

  • Hurd, T.R.; Collins, Y.; Abakumova, I.; Chouchani, E.T.; Baranowski, B.; Fearnley, I.M.; Prime, T.A.; Murphy, M.P.; James, A.M.
    Inactivation of pyruvate dehydrogenase kinase 2 by mitochondrial reactive oxygen species (2012), J. Biol. Chem., 287, 35153-35160.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.2

Cloned(Commentary)

Cloned (Comment) Organism
expression of PDHK2 in HEK293 cells as FLAG-tagged proteins and under a tetracycline-inducible system Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
C195A site-directed mutagenesis Rattus norvegicus
C212A site-directed mutagenesis Rattus norvegicus
C395A site-directed mutagenesis Rattus norvegicus
C45A site-directed mutagenesis Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Dichloroacetate
-
 Rattus norvegicus
H2O2 PDHK2 activity is inhibited by low levels of hydrogen peroxide generated by the respiratory chain via reversible oxidation of Cys45 and Cys392 on PDHK2 Rattus norvegicus
menadione PDHK2 activity is inhibited via reversible oxidation of Cys45 and Cys392 on PDHK2 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 dimeric enzyme, an intermolecular disulfide between two Cys-392 residues is present, in wild-type enzyme and mutant C45A Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Rattus norvegicus
-

Subunits

Subunits Comment Organism
dimer
-
 Rattus norvegicus
monomer
-
 Rattus norvegicus

Synonyms

Synonyms Comment Organism
PDHK2
-
 Rattus norvegicus
pyruvate dehydrogenase kinase 2
-
 Rattus norvegicus

General Information

General Information Comment Organism
malfunction enzyme inhibition results in increased pyruvate dehydrogenase complex activity. Oxidation of Cys45 or Cys392 inhibits PDHK2 activity. Dimer formation is not essential for PDHK2 inactivation via Cys392 oxidation, although it may potentiate it Rattus norvegicus
metabolism PDHK2 is responsible for short-term metabolic responses Rattus norvegicus