Cloned (Comment) | Organism |
---|---|
coexpression of T7-tagged PDK1 and His-tagged PDK2 polypeptides in Escherichia coli, formation of a hybrid dimer | Rattus norvegicus |
expression of isozyme PDK2 as T7-tagged protein in Escherichia coli | Rattus norvegicus |
expression of isozyme PDK2 in Escherichia coli as His-tagged enzyme | Rattus norvegicus |
expression of T7-tagged and His-tagged isozyme PDK1 in Escherichia coli | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | recombinant homodimers of PDK1 and PDK2 and heterodimers of PDK1 + PDK2, synergism with dichloroacetate; recombinant homodimers of PDK1 and PDK2 and heterodimers of PDK1 + PDK2, synergism with dichloroacetate | Rattus norvegicus | |
Dichloroacetate | recombinant homodimers of PDK1 and PDK2 and heterodimers of PDK1 + PDK2, synergism with dichloroacetate; recombinant homodimers of PDK1 and PDK2 and heterodimers of PDK1 + PDK2, synergism with dichloroacetate | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
ATP | recombinant His-tagged isozyme PDK2, 37°C, pH 7.8 | Rattus norvegicus | |
0.034 | - |
ATP | recombinant His-tagged isozyme PDK1, 37°C, pH 7.8 | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
92000 | - |
recombinant hybrid dimer of PDK1 and PDK2, gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [pyruvate dehydrogenase (lipoamide)] | Rattus norvegicus | catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
isozyme PDK2 | - |
Rattus norvegicus | Q63065 | isozyme PDK1 | - |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli as His-tagged isozyme PDK1 | Rattus norvegicus |
recombinant His-tagged PDK2 expressed in Escherichia coli | Rattus norvegicus |
recombinant hybrid dimer of T7-tagged PDK1 and His-tagged PDK2 | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [pyruvate dehydrogenase (lipoamide)] | recombinant hybrid enzyme of PDK1 and PDK2 phosphorylates site 3 with lower activity than the PDK1 homodimer | Rattus norvegicus | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
ATP + [pyruvate dehydrogenase (lipoamide)] | serine phosphorylation site 3 of subunit E1 is exclusively phosphorylated by isozyme PDK1, not by PDK2, which prefers site 1 over site 2 | Rattus norvegicus | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
ATP + [pyruvate dehydrogenase (lipoamide)] | catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 | Rattus norvegicus | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
additional information | binding of homodimers of PDK1 and PDK2, respectively and the heterodimer of PDK1+PDK2 to the pyruvate dehydrogenase complex via dihydrolipoyl transacetylase | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PDK | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Rattus norvegicus |