Literature summary for 2.7.11.2 extracted from

Steussy, C.N.; Popov, K.M.; Bowker-Kinley, M.M.; Sloan, R.B., Jr.; Harris, R.A.; Hamilton, J.A.
Structure of pyruvate dehydrogenase kinase. Novel folding pattern for a serine protein kinase (2001), J. Biol. Chem., 276, 37443-37450.

Search for more literature for 2.7.11.2

Activating Compound

Activating Compound	Comment	Organism	Structure
dihydrolipoyl transacetylase	isozyme PDK2, activation mechanism, binding structure	Rattus norvegicus

Cloned(Commentary)

Cloned (Comment)	Organism
expression of isozyme PDK2 in Escherichia coli as His-tagged protein	Rattus norvegicus

Crystallization (Commentary)

Crystallization (Comment)	Organism
vapor diffusion method, protein 10 mg/ml in 100 mM imidazole or 100 mM MES, pH 6.5-7.0, 0.5 M KCL, 3.5 mM 3-iodopropionic acid, 3.5 mM ADP, 7 mM MgCl2, 6.5% polyethylene glycol 6000, 1.0% ethylene glycol, 1 week, cryoprotection with 25% glycerol, X-ray diffraction structure determination	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Rattus norvegicus	5739	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + [pyruvate dehydrogenase (lipoamide)]	Rattus norvegicus	-	ADP + [pyruvate dehydrogenase (lipoamide)] phosphate	-	ir

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	isozyme PDK2, Protein Data Bank: 1JM6	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant from Escherichia coli BL21(DE3) as His-tagged protein, to homogeneity	Rattus norvegicus

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + [pyruvate dehydrogenase (acetyl-transferring)] = ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate	mechanism	Rattus norvegicus	a
ATP + [pyruvate dehydrogenase (acetyl-transferring)] = ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate	structure of the nucleotide binding pocket with responsible His115 residue and of the catalytic site	Rattus norvegicus	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + [pyruvate dehydrogenase (lipoamide)]	-	Rattus norvegicus	ADP + [pyruvate dehydrogenase (lipoamide)] phosphate	-	ir

Subunits

Subunits	Comment	Organism
dimer	dimer of monomers built of 2 different domains, crystal structure	Rattus norvegicus

Synonyms

Synonyms	Comment	Organism
PDK	-	Rattus norvegicus

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Release 2023.1 (January 2023)