Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dihydrolipoyl transacetylase | isozyme PDK2, activation mechanism, binding structure | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
expression of isozyme PDK2 in Escherichia coli as His-tagged protein | Rattus norvegicus |
Crystallization (Comment) | Organism |
---|---|
vapor diffusion method, protein 10 mg/ml in 100 mM imidazole or 100 mM MES, pH 6.5-7.0, 0.5 M KCL, 3.5 mM 3-iodopropionic acid, 3.5 mM ADP, 7 mM MgCl2, 6.5% polyethylene glycol 6000, 1.0% ethylene glycol, 1 week, cryoprotection with 25% glycerol, X-ray diffraction structure determination | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [pyruvate dehydrogenase (lipoamide)] | Rattus norvegicus | - |
ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
isozyme PDK2, Protein Data Bank: 1JM6 | - |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli BL21(DE3) as His-tagged protein, to homogeneity | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + [pyruvate dehydrogenase (acetyl-transferring)] = ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate | mechanism | Rattus norvegicus | |
ATP + [pyruvate dehydrogenase (acetyl-transferring)] = ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate | structure of the nucleotide binding pocket with responsible His115 residue and of the catalytic site | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [pyruvate dehydrogenase (lipoamide)] | - |
Rattus norvegicus | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir |
Subunits | Comment | Organism |
---|---|---|
dimer | dimer of monomers built of 2 different domains, crystal structure | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
PDK | - |
Rattus norvegicus |