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Literature summary for 2.7.11.2 extracted from

  • Korotchkina, L.G.; Patel, M.S.
    Site specificity of four pyruvate dehydrogenase kinase isoenzymes toward the three phosphorylation sites of human pyruvate dehydrogenase (2001), J. Biol. Chem., 276, 37223-37229.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dihydrolipoyl transacetylase degree of interaction and mechanism differ for the 4 different isozymes Mammalia
dihydrolipoyl transacetylase degree of interaction and mechanism differ for the 4 different isozymes Homo sapiens
dihydrolipoyl transacetylase degree of interaction and mechanism differ for the 4 different isozymes Rattus norvegicus
dihydrolipoyl transacetylase activation in presence of a binding protein, referred to as dihydrolipoamide dehydrogenase-binding protein Homo sapiens
dihydrolipoyl transacetylase activation in presence of a binding protein, referred to as dihydrolipoamide dehydrogenase-binding protein Rattus norvegicus
dihydrolipoyl transacetylase activation depends on the buffer system, the isozyme and the reduction status of the lipoyl groups Homo sapiens
dihydrolipoyl transacetylase activation depends on the buffer system, the isozyme and the reduction status of the lipoyl groups Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of isozyme PDK3 in Escherichia coli BL21(DE3) as His-tagged protein Homo sapiens
overexpression of isozymes PDK1, PDK2, and PDK4 in Escherichia coli BL21(DE3) as His-tagged proteins Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Mammalia
Mg2+
-
Homo sapiens
Mg2+
-
Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [pyruvate dehydrogenase (lipoamide)] Mammalia catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
ir
ATP + [pyruvate dehydrogenase (lipoamide)] Homo sapiens catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
ir
ATP + [pyruvate dehydrogenase (lipoamide)] Rattus norvegicus catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
ir

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
isozyme PDK3
-
Mammalia
-
at least 4 different isozymes: PDK1, PDK2, PDK3, PDK4
-
Rattus norvegicus
-
3 isozymes PDHK1, PDHK2, PDHK4
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information tissue-specific expression, regulation mechanisms Mammalia
-
additional information tissue-specific expression, regulation mechanisms Homo sapiens
-
additional information tissue-specific expression, regulation mechanisms Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activity depends on the buffer system, the reduction status of the lipoyl groups and on the serine phosphorylation site of the E1 subunit of the pyruvate dehydrogenase complex used as substrate Homo sapiens
additional information
-
activity depends on the buffer system, the reduction status of the lipoyl groups and on the serine phosphorylation site of the E1 subunit of the pyruvate dehydrogenase complex used as substrate Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [pyruvate dehydrogenase (lipoamide)] pyruvate dehydrogenase complex substrate is inactivated by ATP-dependent phosphorylation of 3 serine residues on the E1 subunit Mammalia ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
ir
ATP + [pyruvate dehydrogenase (lipoamide)] pyruvate dehydrogenase complex substrate is inactivated by ATP-dependent phosphorylation of 3 serine residues on the E1 subunit Homo sapiens ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
ir
ATP + [pyruvate dehydrogenase (lipoamide)] pyruvate dehydrogenase complex substrate is inactivated by ATP-dependent phosphorylation of 3 serine residues on the E1 subunit Rattus norvegicus ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
ir
ATP + [pyruvate dehydrogenase (lipoamide)] the 3 serine phosphorylation sites of the E1 subunit are specifically and with different activity phosphorylated by the 4 isozymes, overview: site 1 is preferably utilized by PDK2, site 2 by PDK3, and site 3 is exclusively utilized by PDK1 Homo sapiens ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
ir
ATP + [pyruvate dehydrogenase (lipoamide)] the 3 serine phosphorylation sites of the E1 subunit are specifically and with different activity phosphorylated by the 4 isozymes, overview: site 1 is preferably utilized by PDK2, site 2 by PDK3, and site 3 is exclusively utilized by PDK1 Rattus norvegicus ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
ir
ATP + [pyruvate dehydrogenase (lipoamide)] catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 Mammalia ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
ir
ATP + [pyruvate dehydrogenase (lipoamide)] catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 Homo sapiens ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
ir
ATP + [pyruvate dehydrogenase (lipoamide)] catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 Rattus norvegicus ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
ir
additional information activity depends on the buffer system, the reduction status of the lipoyl groups and on the serine phosphorylation site of the E1 subunit of the pyruvate dehydrogenase complex used as substrate Homo sapiens ?
-
?
additional information activity depends on the buffer system, the reduction status of the lipoyl groups and on the serine phosphorylation site of the E1 subunit of the pyruvate dehydrogenase complex used as substrate Rattus norvegicus ?
-
?

Synonyms

Synonyms Comment Organism
PDK
-
Mammalia
PDK
-
Homo sapiens
PDK
-
Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens
30
-
assay at Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0383
-
[Pyruvate dehydrogenase (lipoamide)] phosphorylation site 3 of subunit E1, isozyme PDK1, pH 7.0, 30°C Rattus norvegicus
0.0933
-
[Pyruvate dehydrogenase (lipoamide)] phosphorylation site 2 of subunit E1, isozyme PDK3, pH 7.0, 30°C Rattus norvegicus
0.108
-
[Pyruvate dehydrogenase (lipoamide)] phosphorylation site 2 of subunit E1, isozyme PDK4, pH 7.0, 30°C Rattus norvegicus
0.11
-
[Pyruvate dehydrogenase (lipoamide)] phosphorylation site 1 of subunit E1, isozyme PDK1, pH 7.0, 30°C Rattus norvegicus
0.277
-
[Pyruvate dehydrogenase (lipoamide)] phosphorylation site 1 of subunit E1, isozyme PDK2, pH 7.0, 30°C Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
different buffer systems Homo sapiens
additional information
-
different buffer systems Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP dependent on Mammalia
ATP dependent on Homo sapiens
ATP dependent on Rattus norvegicus