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Literature summary for 2.7.11.2 extracted from

  • Rowles, J.; Scherer, S.W.; Xi, T.; Majer, M.; Nickle, D.C.; Rommens, J.M.; Popov, K.M.; Harris, R.A.; Riebow, N.L.; et al.
    Cloning and characterization of PDK4 on 7q21.3 encoding a fourth pyruvate dehydrogenase kinase isoenzyme in human (1996), J. Biol. Chem., 271, 22376-22382.
    View publication on PubMed
Search for more literature for 2.7.11.2

Cloned(Commentary)

Cloned (Comment) Organism
gene pdk4, DNA and amino sequence determination and analysis, genomic organisation, functional expression of the His-tagged enzyme in Spodoptera frugiperda Sf9 cells via bacuovirus infection system Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activation Homo sapiens
Mg2+
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47500
-
 x * 47500, recombinant His-tagged enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [pyruvate dehydrogenase (lipoamide)] Homo sapiens
-
 ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir
ATP + [pyruvate dehydrogenase (lipoamide)] Homo sapiens catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir

Organism

Organism UniProt Comment Textmining
Homo sapiens Q16654 isozyme PDK4
-
Homo sapiens Q16654 isozyme 4, i.e. PDK4
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged protein from Sf9 insect cells, to near homogeneity Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.008
-
 purified recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [pyruvate dehydrogenase (lipoamide)]
-
 Homo sapiens ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir
ATP + [pyruvate dehydrogenase (lipoamide)] catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 Homo sapiens ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir

Subunits

Subunits Comment Organism
? x * 47500, recombinant His-tagged enzyme, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
PDK
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 room temperature, assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP dependent on Homo sapiens