Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, free pyruvate dehydrogenase kinase, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [pyruvate dehydrogenase (lipoamide)] | Rattus norvegicus | catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
two kinases: pyruvate dehydrogenase-intrinsic activity and free pyruvate dehydrogenase kinase | - |
Rattus norvegicus | - |
4.5fold higher specific activity in starved than in fed rats | - |
Purification (Comment) | Organism |
---|---|
free pyruvate dehydrogenase kinase, separable from pyruvate dehydrogenase complex by gel filtration, to homogeneity | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.276 | - |
fed rat, purified enzyme | Rattus norvegicus |
1.24 | - |
starved rat, purified enzyme | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [pyruvate dehydrogenase (lipoamide)] | - |
Rattus norvegicus | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
ATP + [pyruvate dehydrogenase (lipoamide)] | catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 | Rattus norvegicus | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, free pyruvate dehydrogenase kinase, SDS-PAGE | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Rattus norvegicus |