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Literature summary for 2.7.11.2 extracted from
- Pyruvate dehydrogenase kinase activity of pig heart pyruvate dehydrogenase (E1 component of pyruvate dehydrogenase complex) (1985), Biochem. J., 231, 523-529.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dihydrolipoyl transacetylase | - |
Bos taurus | |
| dihydrolipoyl transacetylase | rate-limiting in the holo-complex | Sus scrofa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| pyruvate | - |
Bos taurus |  |
| pyruvate | - |
Sus scrofa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Sus scrofa | 5739 | - |
| mitochondrion | - |
Bos taurus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Sus scrofa | |
| Mg2+ | requirement | Bos taurus | |
| Mg2+ | actual substrate: MgATP2- | Sus scrofa | |
| Mg2+ | actual substrate: MgATP2- | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 136000 | - |
pyruvate dehydrogenase, gel filtration | Sus scrofa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + [pyruvate dehydrogenase (lipoamide)] | Sus scrofa | catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
| ATP + [pyruvate dehydrogenase (lipoamide)] | Bos taurus | catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sus scrofa |
| kidney, from highly purifed pyruvate dehydrogenase-complex | Bos taurus |
| removed from the dihydrolipoyl transacetylase by treatment with p-hydroxymercuriphenylsulfonate | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Sus scrofa | - |
| heart | - |
Bos taurus | - |
| kidney | - |
Sus scrofa | - |
| kidney | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.9 | 2.7 | purified enzyme, in presence of dihydrolipoyl transacetylase, reconstituted enzyme complex | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + [pyruvate dehydrogenase (lipoamide)] | incorporates gamma-phosphate from ATP into E1-component of pyruvate dehydrogenase-complex alpha-subunit | Sus scrofa | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
| ATP + [pyruvate dehydrogenase (lipoamide)] | incorporates gamma-phosphate from ATP into E1-component of pyruvate dehydrogenase-complex alpha-subunit | Bos taurus | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
| ATP + [pyruvate dehydrogenase (lipoamide)] | catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 | Sus scrofa | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
| ATP + [pyruvate dehydrogenase (lipoamide)] | catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 | Bos taurus | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | subunit composition and complex structure | Sus scrofa |
| More | subunit composition and complex structure | Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Sus scrofa |
| 30 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Sus scrofa |
| 7 | - |
assay at | Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | dependent on | Sus scrofa | |
| ATP | dependent on | Bos taurus | |
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Release 2023.1 (January 2023)
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