Literature summary for 2.7.11.19 extracted from

Owen, D.J.; Papageorgiou, A.C.; Garman, E.F.; Noble, M.E.M.; Johnson, L.N.
Expression, purification and crystallisation of phosphorylase kinase catalytic domain (1995), J. Mol. Biol., 246, 374-381.

Search for more literature for 2.7.11.19

Cloned(Commentary)

Cloned (Comment)	Organism
rabbit muscle phosphorylase kinase catalytic domain of catalytic subunit, expressed in Escherichia coli	Oryctolagus cuniculus

Crystallization (Commentary)

Crystallization (Comment)	Organism
rabbit muscle phosphorylase kinase catalytic domain of catalytic subunit, i.e. Phkgammatrnc, in the presence of Mg-ATP, X-ray data	Oryctolagus cuniculus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.018	-	ATP	phosphorylase b, truncated form of phosphorylase kinase gamma subunit	Oryctolagus cuniculus
0.044	0.08	phosphorylase b	holoenzyme	Oryctolagus cuniculus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	requirement	Oryctolagus cuniculus
Mg2+	major role of Mg2+: cosubstrate in Mg2+-ATP complex	Oryctolagus cuniculus
phosphate	requirement, phosphate containing enzyme	Oryctolagus cuniculus

Organism

Organism	UniProt	Comment	Textmining
Oryctolagus cuniculus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
catalytic subunit as expressed in Escherichia coli	Oryctolagus cuniculus

Source Tissue

Source Tissue	Comment	Organism	Textmining
skeletal muscle	-	Oryctolagus cuniculus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + phosphorylase b	cosubstrate: Mg-ATP complex	Oryctolagus cuniculus	ADP + phosphorylase a	-	?

Subunits

Subunits	Comment	Organism
More	alpha and beta subunits are regulatory subunits controlled by phosphorylation and proteolysis, Ca2+-sensitivity is conferred to delta subunit	Oryctolagus cuniculus
More	the catalytic gamma subunit contains a kinase domain and a calmodulin binding domain	Oryctolagus cuniculus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Oryctolagus cuniculus

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Release 2023.1 (January 2023)