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Literature summary for 2.7.11.19 extracted from
- Phosphorylase kinase from rabbit skeletal muscle (1983), Methods Enzymol., 99, 243-250.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Catalytic subunit of cAMP-dependent protein kinase | activation of nonactivated enzyme | Oryctolagus cuniculus | |
| Catalytic subunit of cAMP-dependent protein kinase | and beta | Oryctolagus cuniculus | |
| Catalytic subunit of cAMP-dependent protein kinase | alpha | Oryctolagus cuniculus | |
| chymotrypsin | proteolytic activation of nonactivated enzyme | Oryctolagus cuniculus | |
| chymotrypsin | by limited proteolysis of alpha subunit, not gamma or delta subunits | Oryctolagus cuniculus | |
| additional information | the nonactivated enzyme is activated either by limited proteolysis | Oryctolagus cuniculus | |
| Trypsin | proteolytic activation of nonactivated enzyme | Oryctolagus cuniculus | |
| Trypsin | strong, by limited proteolysis of alpha and beta subunits (not gamma) | Oryctolagus cuniculus | |
| Trypsin | strong, by limited proteolysis of alpha and beta subunits | Oryctolagus cuniculus | |
| Trypsin | strong, by limited proteolysis of alpha and beta subunits or delta subunits | Oryctolagus cuniculus |
General Stability
| General Stability | Organism |
|---|---|
| With strong tendency to aggregate, unstable in high concentrations of ammonium sulfate for prolonged periods | Oryctolagus cuniculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
ATP | - |
Oryctolagus cuniculus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| glycogen particle | - |
Oryctolagus cuniculus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Oryctolagus cuniculus | |
| Mg2+ | requirement | Oryctolagus cuniculus | |
| Mg2+ | major role of Mg2+: cosubstrate in Mg2+-ATP complex | Oryctolagus cuniculus | |
| phosphate | requirement, phosphate containing enzyme | Oryctolagus cuniculus | |
| phosphate | alpha and beta subunits are phosphorylated by protein kinases or autophosphorylation | Oryctolagus cuniculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17000 | - |
4 * 145000 + 4 * 130000 + 4 * 45000 + 4 * 17000, (alphabetagammadelta)4, rabbit, SDS-PAGE | Oryctolagus cuniculus |
| 45000 | - |
4 * 145000 + 4 * 130000 + 4 * 45000 + 4 * 17000, (alphabetagammadelta)4, rabbit, SDS-PAGE | Oryctolagus cuniculus |
| 130000 | - |
4 * 145000 + 4 * 130000 + 4 * 45000 + 4 * 17000, (alphabetagammadelta)4, rabbit, SDS-PAGE | Oryctolagus cuniculus |
| 145000 | - |
4 * 145000 + 4 * 130000 + 4 * 45000 + 4 * 17000, (alphabetagammadelta)4, rabbit, SDS-PAGE | Oryctolagus cuniculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + glycogen synthase | Oryctolagus cuniculus | conversion to a glucose 6-phosphate dependent form | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
New Zealand white (female) | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from protein-glycogen complex | Oryctolagus cuniculus |
| to near homogeneity | Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| skeletal muscle | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.2 | - |
- |
Oryctolagus cuniculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, in 50 mM sodium glycerophosphate, 0.1% v/v 2-mercaptoethanol, pH 7, 2 mM EDTA, 50% v/v glycerol, at least 1 year | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + glycogen synthase | conversion to a glucose 6-phosphate dependent form | Oryctolagus cuniculus | ? | - |
? | |
| ATP + glycogen synthase | glycogen synthase a | Oryctolagus cuniculus | ADP + phosphoglycogen synthase | - |
? | |
| ATP + glycogen synthase | phosphorylatable residue: Ser-7 | Oryctolagus cuniculus | ADP + phosphoglycogen synthase | - |
? | |
| ATP + glycogen synthase | at the same rate as phosphorylase b | Oryctolagus cuniculus | ADP + phosphoglycogen synthase | - |
? | |
| ATP + phosphorylase b | cosubstrate: Mg-ATP complex | Oryctolagus cuniculus | ADP + phosphorylase a | - |
? | |
| ATP + phosphorylase b | phosphorylation site: Ser-14 | Oryctolagus cuniculus | ADP + phosphorylase a | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexadecamer | 4 * 145000 + 4 * 130000 + 4 * 45000 + 4 * 17000, (alphabetagammadelta)4, rabbit, SDS-PAGE | Oryctolagus cuniculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Oryctolagus cuniculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity ratios pH 6.8/8.2 of nonactivated enzyme: 0.07 (in the presence of 0.05-0.07 M Ca2+) and 0.23 (after calmodulin addition) | Oryctolagus cuniculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Calmodulin | additional activation | Oryctolagus cuniculus | |
| Calmodulin | no activation of phosphorylated or proteolytically modified enzyme by delta'-subunit | Oryctolagus cuniculus | |
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