Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Catalytic subunit of cAMP-dependent protein kinase | activation of nonactivated enzyme | Bos taurus | |
glycogen | activation | Bos taurus | |
glycogen | effect on nonactivated and activated enzyme | Bos taurus | |
additional information | the nonactivated enzyme is activated either by limited proteolysis | Bos taurus | |
Trypsin | proteolytic activation of nonactivated enzyme | Bos taurus | |
Trypsin | increase of pH 6.8 activity, not pH 8.2 activity | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | influence on kinetic parameters, glycogen decreases Km-values for phosphorylase b | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Bos taurus | |
Mg2+ | major role of Mg2+: cosubstrate in Mg2+-ATP complex | Bos taurus | |
phosphate | requirement, phosphate containing enzyme | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
MW of trypsinized enzyme | Bos taurus |
18000 | - |
4 * 140000 + 4 * 130000 + 4 * 46000 + 4 * 18000, (alphabetagammadelta)4, SDS-PAGE | Bos taurus |
46000 | - |
4 * 140000 + 4 * 130000 + 4 * 46000 + 4 * 18000, (alphabetagammadelta)4, SDS-PAGE | Bos taurus |
130000 | - |
4 * 140000 + 4 * 130000 + 4 * 46000 + 4 * 18000, (alphabetagammadelta)4, SDS-PAGE | Bos taurus |
140000 | - |
4 * 140000 + 4 * 130000 + 4 * 46000 + 4 * 18000, (alphabetagammadelta)4, SDS-PAGE | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nonactivated enzyme | Bos taurus |
to near homogeneity | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cardiac muscle | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.3 | - |
- |
Bos taurus |
Storage Stability | Organism |
---|---|
Frozen in liquid N2, in 50 mM beta-glycerophosphate, pH 7, 2 mM EDTA, 1 mM DTT, 10% sucrose, stable | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + phosphorylase b | cosubstrate: Mg-ATP complex | Bos taurus | ADP + phosphorylase a | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexadecamer | 4 * 140000 + 4 * 130000 + 4 * 46000 + 4 * 18000, (alphabetagammadelta)4, SDS-PAGE | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
nonactivated enzyme has only low activity at pH 6.8 | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | no additional activation | Bos taurus |