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Literature summary for 2.7.11.19 extracted from
- Purification and partial characterization of bovine heart phosphorylase kinase (1983), Arch. Biochem. Biophys., 221, 333-342.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Catalytic subunit of cAMP-dependent protein kinase | activation of nonactivated enzyme | Bos taurus | |
| glycogen | activation | Bos taurus |  |
| glycogen | effect on nonactivated and activated enzyme | Bos taurus | |
| additional information | the nonactivated enzyme is activated either by limited proteolysis | Bos taurus | |
| Trypsin | proteolytic activation of nonactivated enzyme | Bos taurus | |
| Trypsin | increase of pH 6.8 activity, not pH 8.2 activity | Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | influence on kinetic parameters, glycogen decreases Km-values for phosphorylase b | Bos taurus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Bos taurus | |
| Mg2+ | major role of Mg2+: cosubstrate in Mg2+-ATP complex | Bos taurus | |
| phosphate | requirement, phosphate containing enzyme | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
MW of trypsinized enzyme | Bos taurus |
| 18000 | - |
4 * 140000 + 4 * 130000 + 4 * 46000 + 4 * 18000, (alphabetagammadelta)4, SDS-PAGE | Bos taurus |
| 46000 | - |
4 * 140000 + 4 * 130000 + 4 * 46000 + 4 * 18000, (alphabetagammadelta)4, SDS-PAGE | Bos taurus |
| 130000 | - |
4 * 140000 + 4 * 130000 + 4 * 46000 + 4 * 18000, (alphabetagammadelta)4, SDS-PAGE | Bos taurus |
| 140000 | - |
4 * 140000 + 4 * 130000 + 4 * 46000 + 4 * 18000, (alphabetagammadelta)4, SDS-PAGE | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nonactivated enzyme | Bos taurus |
| to near homogeneity | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cardiac muscle | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.3 | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| Frozen in liquid N2, in 50 mM beta-glycerophosphate, pH 7, 2 mM EDTA, 1 mM DTT, 10% sucrose, stable | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + phosphorylase b | cosubstrate: Mg-ATP complex | Bos taurus | ADP + phosphorylase a | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexadecamer | 4 * 140000 + 4 * 130000 + 4 * 46000 + 4 * 18000, (alphabetagammadelta)4, SDS-PAGE | Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
nonactivated enzyme has only low activity at pH 6.8 | Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Calmodulin | no additional activation | Bos taurus | |
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