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Literature summary for 2.7.11.19 extracted from

  • Pocinwong, S.; Blum, H.; Malencik, D.; Fisher, E.H.
    Phosphorylase kinase from dogfish skeletal muscle. Purification and properties (1981), Biochemistry, 20, 7219-7226.
    View publication on PubMed
Search for more literature for 2.7.11.19

Activating Compound

Activating Compound Comment Organism Structure
Catalytic subunit of cAMP-dependent protein kinase not Oryctolagus cuniculus
Catalytic subunit of cAMP-dependent protein kinase not Squalus acanthias
glycogen no significant effect on dogfish enzyme Squalus acanthias
additional information no activation by glucose 6-phosphate, UDPglucose, dogfish myosin, actin, tropomyosin, rabbit glycogen synthase Oryctolagus cuniculus
additional information no activation by glucose 6-phosphate, UDPglucose, dogfish myosin, actin, tropomyosin, rabbit glycogen synthase Squalus acanthias
additional information No activation by substrates of phosphorylase b reaction, i.e. AMP or glucose 1-phosphate Oryctolagus cuniculus
additional information No activation by substrates of phosphorylase b reaction, i.e. AMP or glucose 1-phosphate Squalus acanthias
additional information no activation by parvalbumin Oryctolagus cuniculus
additional information no activation by parvalbumin Squalus acanthias
Protein kinases not Squalus acanthias

General Stability

General Stability Organism
Ca2+-free enzyme is unstable Squalus acanthias
Rapid irreversible inactivation during desalting by gel filtration or dialysis at ionic strength below 0.1, sucrose, glycerol, salts, SH-compounds or reagents does not stabilize, glycogen, glucose, glucose 1-phosphate, glucose 6-phosphate, mono-, di- and trinucleotides or divalent metal ions and protease inhibitors do not protect Squalus acanthias
Tends to aggregate upon standing Squalus acanthias

Inhibitors

Inhibitors Comment Organism Structure
EDTA Ca2+ restores Oryctolagus cuniculus
EDTA Ca2+ restores Squalus acanthias
EGTA Ca2+ restores; strong Oryctolagus cuniculus
EGTA Ca2+ restores; strong Squalus acanthias
Protein phosphatase rabbit (not dogfish) kinase Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.084
-
 phosphorylase b from rabbit, pH 8.2, dogfish enzyme Squalus acanthias
0.1
-
 MgATP2- dogfish phosphorylase b, pH 8.2, dogfish enzyme Squalus acanthias

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Oryctolagus cuniculus 5829
-
cytosol
-
 Squalus acanthias 5829
-
soluble
-
 Oryctolagus cuniculus
-
-
soluble
-
 Squalus acanthias
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+
-
 Oryctolagus cuniculus
Ba2+ activation, can replace Ca2+ with 26% efficiency Squalus acanthias
Ca2+
-
 Oryctolagus cuniculus
Ca2+ requirement Squalus acanthias
Co2+ activation, can replace Ca2+ with 10% efficiency Squalus acanthias
Fe3+ activation, can replace Ca2+ with 10% efficiency Squalus acanthias
Li+ activation Oryctolagus cuniculus
Li+ activation Squalus acanthias
Li+ can replace Ca2+ with 10% efficiency Squalus acanthias
Mg2+ requirement Oryctolagus cuniculus
Mg2+ requirement Squalus acanthias
Mg2+ major role of Mg2+: cosubstrate in Mg2+-ATP complex Oryctolagus cuniculus
Mg2+ major role of Mg2+: cosubstrate in Mg2+-ATP complex Squalus acanthias
Mn2+ requirement Oryctolagus cuniculus
Mn2+ requirement Squalus acanthias
Mn2+ can replace Ca2+ with 15% efficiency Oryctolagus cuniculus
Mn2+ can replace Ca2+ with 15% efficiency Squalus acanthias
additional information no activation by Cu2+, Cd2+, Sn2+, Al3+ Oryctolagus cuniculus
additional information no activation by Cu2+, Cd2+, Sn2+, Al3+ Squalus acanthias
additional information Fe2+, Zn2+ or Ni2+ Oryctolagus cuniculus
additional information Fe2+, Zn2+ or Ni2+ Squalus acanthias
phosphate requirement, phosphate containing enzyme Oryctolagus cuniculus
phosphate requirement, phosphate containing enzyme Squalus acanthias
Sr2+ activation, can replace Ca2+ with 45% efficiency Oryctolagus cuniculus
Sr2+ activation, can replace Ca2+ with 45% efficiency Squalus acanthias

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1300000
-
 dogfish, gel filtration or sedimentation velocity analysis Oryctolagus cuniculus
1300000
-
 dogfish, gel filtration or sedimentation velocity analysis Squalus acanthias

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-
Squalus acanthias
-
 i.e. Pacific dogfish or Squalus acanthias
-

Purification (Commentary)

Purification (Comment) Organism
-
 Squalus acanthias

Source Tissue

Source Tissue Comment Organism Textmining
additional information
-
 Oryctolagus cuniculus
-
additional information tissue distribution in dogfish Squalus acanthias
-
skeletal muscle
-
 Oryctolagus cuniculus
-
skeletal muscle
-
 Squalus acanthias
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8
-
 dogfish Squalus acanthias

Storage Stability

Storage Stability Organism
-15°C, 3 mg dogfish enzyme/ml, in 0.1 M glycerophosphate, 2 mM EDTA, pH 7, 0.3 M NaCl or in 0.1 M glycerophosphate, 2 mM EDTA, 10% sucrose, 1 mM ATP, pH 7, 1 mM DTT, about 50% loss of activity within 1 week Squalus acanthias
-15°C, partially purified dogfish enzyme, lyophilized, almost indefinitely Squalus acanthias

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + nonactivated phosphorylase kinase i.e. autophosphorylation and autoactivation Oryctolagus cuniculus ADP + activated phosphorylase kinase
-
 ?
ATP + nonactivated phosphorylase kinase i.e. autophosphorylation and autoactivation Squalus acanthias ADP + activated phosphorylase kinase
-
 ?
ATP + nonactivated phosphorylase kinase ATP can be replaced by dATP or adenosine 5'-(3-thiotriphosphate) with 50% and 10% efficiency, respectively Squalus acanthias ADP + activated phosphorylase kinase
-
 ?
ATP + phosphorylase b cosubstrate: Mg-ATP complex Oryctolagus cuniculus ADP + phosphorylase a
-
 ?
ATP + phosphorylase b cosubstrate: Mg-ATP complex Squalus acanthias ADP + phosphorylase a
-
 ?
ATP + phosphorylase b dogfish Squalus acanthias ADP + phosphorylase a
-
 ?
ATP + phosphorylase b rabbit skeletal muscle Oryctolagus cuniculus ADP + phosphorylase a
-
 ?
ATP + phosphorylase b rabbit skeletal muscle Squalus acanthias ADP + phosphorylase a
-
 ?
ATP + synthetic tetradecapeptide i.e. Ser-Asp-Gln-Glu-Lys-Arg-Lys-Gln-Ile-Ser-Val-Arg-Gly-Leu Oryctolagus cuniculus ?
-
 ?
ATP + synthetic tetradecapeptide i.e. Ser-Asp-Gln-Glu-Lys-Arg-Lys-Gln-Ile-Ser-Val-Arg-Gly-Leu Squalus acanthias ?
-
 ?
ATP + synthetic tetradecapeptide phosphorylation site: Ser between Ile and Val Squalus acanthias ?
-
 ?
ATP + troponin I not rabbit or dogfish troponin I Oryctolagus cuniculus ADP + phosphotroponin I
-
 ?
ATP + troponin I not rabbit or dogfish troponin I Squalus acanthias ADP + phosphotroponin I
-
 ?
ATP + troponin T not rabbit or dogfish troponin T Oryctolagus cuniculus ADP + phosphotroponin T
-
 ?
ATP + troponin T not rabbit or dogfish troponin T Squalus acanthias ADP + phosphotroponin T
-
 ?
GTP + phosphorylase b not (dogfish) Squalus acanthias GDP + phosphorylase a
-
 ?
additional information creatine phosphate, phosphoenolpyruvate, actin, parvalbumin, protamin, dogfish or rabbit myosin, adenosine 5'-(3-methyltriphosphate), 5'-adenylylimidodiphosphate (dogfish) are no substrates Oryctolagus cuniculus ?
-
 ?
additional information creatine phosphate, phosphoenolpyruvate, actin, parvalbumin, protamin, dogfish or rabbit myosin, adenosine 5'-(3-methyltriphosphate), 5'-adenylylimidodiphosphate (dogfish) are no substrates Squalus acanthias ?
-
 ?
additional information ITP, and CTP are no substrates Oryctolagus cuniculus ?
-
 ?
additional information ITP, and CTP are no substrates Squalus acanthias ?
-
 ?
additional information histone II-A is no substrate Oryctolagus cuniculus ?
-
 ?
additional information histone II-A is no substrate Squalus acanthias ?
-
 ?
additional information phosvitin is no substrate Oryctolagus cuniculus ?
-
 ?
additional information phosvitin is no substrate Squalus acanthias ?
-
 ?
UTP + phosphorylase b not (dogfish) Squalus acanthias UDP + phosphorylase a
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Oryctolagus cuniculus
30
-
 assay at Squalus acanthias

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Squalus acanthias

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 progressive increase of activity, biphasic with dogfish phosphorylase b as substrate: then about half-maximal activity at pH 7.2-7.6 (dogfish activated enzyme) Squalus acanthias
6.8 8.5 about half-maximal activity at pH 6.8 and maximal activity at pH 8.5, activated rabbit enzyme Oryctolagus cuniculus
8.1 8.5 about half-maximal activity at pH 8.1 and maximal activity at pH 8.5, nonactivated rabbit enzyme Oryctolagus cuniculus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
 below, rapid irreversible inactivation Oryctolagus cuniculus
6
-
 below, rapid irreversible inactivation Squalus acanthias