Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.11.19 extracted from

  • Skuster, J.R.; Chan, K.F.J.; Graves, D.J.
    Isolation and properties of the catalytically active gamma subunit of phosphorylase b kinase (1980), J. Biol. Chem., 255, 2203-2210.
    View publication on PubMed
Search for more literature for 2.7.11.19

General Stability

General Stability Organism
Incubation of nonactivated enzyme with 100 mM ATP at 0°C dissociates the 23 S enzyme to active 7.5 S and 14 S subunits, with LiBr it produces 5 S subunits Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
EGTA not Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic parameters of catalytically active gamma subunit at pH 6.8 and 8.5 Oryctolagus cuniculus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stabilization, no absolute requirement for catalytic subunit gamma2 Oryctolagus cuniculus
Mg2+ requirement Oryctolagus cuniculus
Mg2+ major role of Mg2+: cosubstrate in Mg2+-ATP complex Oryctolagus cuniculus
phosphate requirement, phosphate containing enzyme Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 2 * 45000, rabbit, catalytically active gammagamma subunit, SDS-PAGE Oryctolagus cuniculus
86000
-
 catalytically active gammagamma subunit complex, gel filtration Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
catalytic subunit (i.e. gamma subunit, from holoenzyme by dissociation) Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
skeletal muscle
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + nonactivated phosphorylase kinase i.e. autophosphorylation and autoactivation Oryctolagus cuniculus ADP + activated phosphorylase kinase
-
 ?
ATP + phosphorylase b cosubstrate: Mg-ATP complex Oryctolagus cuniculus ADP + phosphorylase a
-
 ?
ATP + synthetic tetradecapeptide from amino-terminal of phosphorylase b Oryctolagus cuniculus ?
-
 ?
additional information no substrates are phosphorylase kinase gamma subunit Oryctolagus cuniculus ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 45000, rabbit, catalytically active gammagamma subunit, SDS-PAGE Oryctolagus cuniculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Oryctolagus cuniculus

pH Range

pH Minimum pH Maximum Comment Organism
6.2 9.5 about half-maximal activity at pH 6.2 and about 70% of maximal activity at pH 9.5 Oryctolagus cuniculus