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Literature summary for 2.7.11.19 extracted from

  • Sakai, K.; Matsumura, S.; Okimura, Y.; Yamamura, H.; Nishizuka, Y.
    Liver glycogen phosphorylase kinase. Partial purification and characterization (1979), J. Biol. Chem., 254, 6631-6637.
    View publication on PubMed
Search for more literature for 2.7.11.19

Activating Compound

Activating Compound Comment Organism Structure
Protein kinases not (liver enzyme) Oryctolagus cuniculus

General Stability

General Stability Organism
About 70% loss of activity during centrifugation for 5 h on a glycerol density gradient Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4 above 0.2 M, stimulates at 0.05-0.1 M Oryctolagus cuniculus
EGTA Ca2+ restores; irreversible upon prolonged incubation (liver enzyme); kinetics Oryctolagus cuniculus
KCl not Oryctolagus cuniculus
NaCl 0.1 M Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic data of holoenzyme and catalytically active proteolytic fragment Oryctolagus cuniculus
0.025
-
 ATP proteolytic fragment of phosphorylase kinase Oryctolagus cuniculus
0.03 0.037 ATP
-
 Oryctolagus cuniculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Oryctolagus cuniculus 5829
-
soluble
-
 Oryctolagus cuniculus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
(NH4)2SO4 activation, 0.05-0.1 M, inhibits above 0.2 M Oryctolagus cuniculus
Ca2+
-
 Oryctolagus cuniculus
Mg2+ requirement Oryctolagus cuniculus
Mg2+ major role of Mg2+: cosubstrate in Mg2+-ATP complex Oryctolagus cuniculus
phosphate requirement, phosphate containing enzyme Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1300000
-
 gel filtration Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
 New Zealand white (male)
-

Purification (Commentary)

Purification (Comment) Organism
catalytically active proteolytic product of holoenzyme Oryctolagus cuniculus
liver Oryctolagus cuniculus
to near homogeneity Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Oryctolagus cuniculus
-
skeletal muscle
-
 Oryctolagus cuniculus
-

Storage Stability

Storage Stability Organism
-25°C, in 50% ethylene glycol, at least 1 month Oryctolagus cuniculus
0-4°C, in 5-20% glycerol, 70% loss of activity within 5 h, more rapid inactivation in 5-20% sucrose Oryctolagus cuniculus
0°C, in 10% ethylene glycol, at least 1 week Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + liver dephosphophosphorylase
-
 Oryctolagus cuniculus ?
-
 ?
ATP + nonactivated phosphorylase kinase i.e. autophosphorylation and autoactivation Oryctolagus cuniculus ADP + activated phosphorylase kinase
-
 ?
ATP + phosphorylase b i.e. EC 2.4.1.1 or glycogen phosphorylase Oryctolagus cuniculus ADP + phosphorylase a
-
 ?
ATP + phosphorylase b cosubstrate: Mg-ATP complex Oryctolagus cuniculus ADP + phosphorylase a
-
 ?
ATP + phosphorylase b incorporation of terminal phosphate of ATP into phosphorylase b Oryctolagus cuniculus ADP + phosphorylase a
-
 ?
ATP + phosphorylase b rabbit skeletal muscle Oryctolagus cuniculus ADP + phosphorylase a
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Oryctolagus cuniculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 at least 30 min Oryctolagus cuniculus
40
-
 t1/2: 3 min, in 10% ethylene glycol, pH 8 Oryctolagus cuniculus
45
-
 t1/2: 1 min, in 10% ethylene glycol, pH 8 Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 liver enzyme Oryctolagus cuniculus
9.5
-
 muscle enzyme Oryctolagus cuniculus

pH Range

pH Minimum pH Maximum Comment Organism
6 11.5 about half-maximal activity at pH 6 and 11.5, liver enzyme Oryctolagus cuniculus
9 10 about half-maximal activity at pH 9 and 10, muscle enzyme Oryctolagus cuniculus