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Literature summary for 2.7.11.19 extracted from

  • Graves, D.J.; Hayakawa, T.; Horvitz, R.A.; Beckman, E.; Krebs, E.G.
    Studies on the subunit structure of trypsin-activated phosphorylase kinase (1973), Biochemistry, 12, 580-585.
    View publication on PubMed
Search for more literature for 2.7.11.19

Activating Compound

Activating Compound Comment Organism Structure
additional information the nonactivated enzyme is activated either by limited proteolysis Oryctolagus cuniculus
Trypsin proteolytic activation of nonactivated enzyme Oryctolagus cuniculus

General Stability

General Stability Organism
Effects of protein concentration, buffer and ATP on stability and dissociation behaviour of trypsin-activated enzyme Oryctolagus cuniculus
In the presence of ATP nonactivated enzyme does not dissociate into catalytically active subunits as trypsin-activated enzyme does Oryctolagus cuniculus
Nonactivated or protein kinase-activated enzyme stable in the cold, not trypsin-activated enzyme Oryctolagus cuniculus
unstable in dilute solutions Oryctolagus cuniculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Oryctolagus cuniculus
Mg2+ major role of Mg2+: cosubstrate in Mg2+-ATP complex Oryctolagus cuniculus
phosphate requirement, phosphate containing enzyme Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
skeletal muscle
-
 Oryctolagus cuniculus
-

Storage Stability

Storage Stability Organism
0°C, 2 mg enzyme/ml, trypsin-activated enzyme, with or without ATP, 30% loss of activity within 4 h Oryctolagus cuniculus
20°C, 2 mg enzyme/ml, trypsin-activated enzyme, with or without ATP, 4 h Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + phosphorylase b cosubstrate: Mg-ATP complex Oryctolagus cuniculus ADP + phosphorylase a
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 2 mg enzyme/ml, trypsin-activated enzyme, with or without ATP, 4 h stable Oryctolagus cuniculus