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Literature summary for 2.7.11.19 extracted from

  • Meyer, W.L.; Fischer, E.H.; Krebs, E.G.
    Activation of skeletal muscle phosphorylase b kinase by calcium ion (1964), Biochemistry, 3, 1033-1039.
    View publication on PubMed
Search for more literature for 2.7.11.19

Activating Compound

Activating Compound Comment Organism Structure
Ca2+-dependent protease i.e. kinase-activating factor Oryctolagus cuniculus
additional information no activation of nonactivated enzyme by renin (with or without Ca2+), thrombin (with or without Ca2+), phospholipase D from Clostridium perfringens or phospholipase from Crotalus adamanteus Oryctolagus cuniculus
additional information the nonactivated enzyme is activated either by limited proteolysis Oryctolagus cuniculus
Trypsin proteolytic activation of nonactivated enzyme Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
EDTA Ca2+ restores; less effective than EGTA Oryctolagus cuniculus
EGTA nonactivated enzyme, more effective than EDTA; strong Oryctolagus cuniculus
additional information no inhibition by diethyldithiocarbamic acid, 2,2'-dipyridyl Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03 0.037 ATP phosphorylase b Oryctolagus cuniculus
0.03 0.037 ATP activated enzyme, pH 7.5 Oryctolagus cuniculus
0.14
-
 phosphorylase b before activation, pH 7.5 Oryctolagus cuniculus

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ activation, can replace Ca2+ with 60% efficiency Oryctolagus cuniculus
Ca2+ irreversible activation of nonactivated kinase by preincubation together with a separate kinase-activating factor independent of cAMP, kinetics Oryctolagus cuniculus
Co2+ not Oryctolagus cuniculus
Fe3+ not Oryctolagus cuniculus
Mg2+ requirement Oryctolagus cuniculus
Mg2+ not Oryctolagus cuniculus
Mg2+ major role of Mg2+: cosubstrate in Mg2+-ATP complex Oryctolagus cuniculus
Mn2+ requirement Oryctolagus cuniculus
Mn2+ can substitute for Ca2+ Oryctolagus cuniculus
additional information Fe2+, Zn2+ or Ni2+ Oryctolagus cuniculus
phosphate requirement, phosphate containing enzyme Oryctolagus cuniculus
Sr2+ activation, can replace Ca2+ with 60% efficiency Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
skeletal muscle
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + phosphorylase b cosubstrate: Mg-ATP complex Oryctolagus cuniculus ADP + phosphorylase a
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Oryctolagus cuniculus