Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | absolutely dependent on | Homo sapiens | |
ionomycin | activates the Ca2+/calmodulin-activated CaM-K1delta further 1.5fold | Homo sapiens | |
additional information | CaM-K1delta is activated about 30fold by phosphorylation through the Ca2+/calmodulin-dependent protein kinase kinase CaM-KKalpha | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
CaM-K1delta, DNA and amino acid sequence determination and analysis, expression of GST-tagged wild-type and mutant enzyme in Escherichia coli strain JM109, transient expression of HA-tagged CaM-K1delta 1-296 Ca2+/calmodulin-independent mutant in HeLa and COS-7 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | CaM-K1delta 1-296 truncation mutant is catalytically active and Ca2+/calmodulin-independent, lacks the putative autoinhibitory domain and the Ca2+/calmodulin binding region, the truncation mutant is fully activated by CaM-KKalpha | Homo sapiens |
T180A | site-directed mutagenesis, the mutant enzyme shows highly reduced activity compared to the wild-type enzyme, the mutant is phosphorylated and activated by CaM-KKalpha | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
STO-609 | the CaM-KKalpha inhibitor STO-609 inhibits activity of the CaM-K1delta truncation mutant 1-296 | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | absolutely dependent on | Homo sapiens | |
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | components of the Ca2+/calmodulin-dependent protein kinase cascade, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8IU85 | isozyme CaM-K1delta | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | CaM-K1delta is activated about 30fold by phosphorylation through the Ca2+/calmodulin-dependent protein kinase kinase CaM-KKalpha, mechanism, CaM-K1delta performs autophosphorylation in a Ca2+/calmodulin-dependent manner which has no impact on its kinase activity | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged wild-type and mutant CaM-K1delta from Escherichia coli by glutathione affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
colon | - |
Homo sapiens | - |
HeLa cell | - |
Homo sapiens | - |
liver | - |
Homo sapiens | - |
additional information | CaM-K1delta tissue expression pattern, no expression in intestine, kidney, placenta, heart, and lung | Homo sapiens | - |
ovary | - |
Homo sapiens | - |
pancreas | - |
Homo sapiens | - |
prostate gland | low expression level of CaM-K1delta | Homo sapiens | - |
skeletal muscle | low expression level of CaM-K1delta | Homo sapiens | - |
spleen | - |
Homo sapiens | - |
testis | high expression level of CaM-K1delta | Homo sapiens | - |
thymus | low expression level of CaM-K1delta | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.035 | - |
purified recombinant CaM-K1delta | Homo sapiens |
0.98 | - |
purified recombinant CaM-K1delta phosphorylated by CaM-KKalpha | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + syntide-2 | - |
Homo sapiens | ADP + phosphorylated syntide-2 | - |
? | |
additional information | components of the Ca2+/calmodulin-dependent protein kinase cascade, overview | Homo sapiens | ? | - |
? | |
additional information | CaM-K1delta performs autophosphorylation in a Ca2+/calmodulin-dependent manner which has no impact on its kinase activity | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CaM-K1delta | - |
Homo sapiens |
CaM-KKbeta3 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |