Activating Compound | Comment | Organism | Structure |
---|---|---|---|
CSF-1 | CSF-1 increases PKCgamma Thr 410 phosphorylation and kinase activity in 32D.R cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bisindolylmaleimide I | - |
Homo sapiens | |
Gö 6983 | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
myeloid cell line | myeloid progenitor cell line 32D.R | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a protein | - |
Homo sapiens | ADP + a phosphoprotein | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PKC-zeta | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0025 | - |
pH not specified in the publication, 30°C | Homo sapiens | Gö 6983 | |
0.0075 | - |
pH not specified in the publication, 30°C | Homo sapiens | bisindolylmaleimide I |
General Information | Comment | Organism |
---|---|---|
physiological function | PKCzeta kinase inhibition reduces CSF-1 supported mitogenesis in 32D.R cells. Its overexpression increases CSF-1 mitogenic responsiveness. PKCzeta's promotion of mitogenic signaling in 32D.R cells is independent of NF-kB. In primary murine bone marrow derived macrophages (BMMs), PKCzeta inhibition has a more modest effect on CSF-1 dependent mitogenesis, and, pan-PKC inhibition has a paradoxically enhancing effect on MEK-Erk phosphorylation. Thus the importance of PKCzeta in the control of CSF-1 mediated MEKErk activity and mitogenesis depends on differentiation stage | Homo sapiens |