DELTA621-622 |
deletion of two residues in the AGC linker that passes through the cleft of the C1B domain (DELTA621622) results in a 2fold decrease in the concentration of phorbol 12-myristate 13-acetate required to mediate half-maximal translocation |
Rattus norvegicus |
F629D |
within the NFD helix, mutant F629D and mutant F633D lead to 2- to 5fold decrease in EC50 for phorbol 12-myristate 13-acetate induced translocation |
Rattus norvegicus |
F633D |
within the NFD helix, mutant F629D and mutant F633D lead to 2- to 5fold decrease in EC50 for phorbol 12-myristate 13-acetate induced translocation |
Rattus norvegicus |
L125D |
mutation of Leu125 in the C1B domain, which is part of the C1B clamp interface, increases EC50 for phorbol 12-myristate 13-acetate induced translocation more than 10fold |
Rattus norvegicus |
L358D |
mutation of residues in the hydrophobic interface between the kinase N lobe and the C1B domain (L358D, L367D, Y422D, Y430D) results in 5- to 14fold decrease in the EC50 for phorbol 12-myristate 13-acetate induced translocation |
Rattus norvegicus |
L367D |
mutation of residues in the hydrophobic interface between the kinase N lobe and the C1B domain (L358D, L367D, Y422D, Y430D) results in 5- to 14fold decrease in the EC50 for phorbol 12-myristate 13-acetate induced translocation |
Rattus norvegicus |
Y422D |
mutation of residues in the hydrophobic interface between the kinase N lobe and the C1B domain (L358D, L367D, Y422D, Y430D) results in 5- to 14fold decrease in the EC50 for phorbol 12-myristate 13-acetate induced translocation |
Rattus norvegicus |
Y430D |
mutation of residues in the hydrophobic interface between the kinase N lobe and the C1B domain (L358D, L367D, Y422D, Y430D) results in 5- to 14fold decrease in the EC50 for phorbol 12-myristate 13-acetate induced translocation |
Rattus norvegicus |