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Literature summary for 2.7.11.13 extracted from

  • Rahman, G.M.; Shanker, S.; Lewin, N.E.; Kedei, N.; Hill, C.S.; Prasad, B.V.; Blumberg, P.M.; Das, J.
    Identification of the activator-binding residues in the second cysteine-rich regulatory domain of protein kinase Ctheta (PKCtheta) (2013), Biochem. J., 451, 33-44.
    View publication on PubMedView publication on EuropePMC
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Activating Compound

Activating Compound Comment Organism Structure
1,2-dioctanoyl-sn-glycerol
-
 Mus musculus
1-stearoyl-2-arachidonoyl-sn-glycerol
-
 Mus musculus
phorbol 12,13-dibutyrate
-
 Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a GST-fusion protein Mus musculus

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of PKCthetaC1B is determined to 1.63 A resolution. It is shown that, Trp253 at the rim of the activator-binding pocket is orientated towards the membrane Mus musculus

Protein Variants

Protein Variants Comment Organism
L255G mutated activator-binding site: mutation reduces affinity for both phorbol ester and diacylglycerol. Mutation causes significantly reduced membrane translocation by phorbol ester treatment Mus musculus
Q258G mutated activator-binding site: mutation reduces affinity for both phorbol ester and diacylglycerol. Mutation causes significantly reduced membrane translocation by phorbol ester treatment Mus musculus
T243A mutated activator-binding site: mutation reduces affinity for both phorbol ester and diacylglycerol. Mutation causes significantly reduced membrane translocation by phorbol ester treatment Mus musculus
W253G mutated activator-binding site: mutation reduces affinity for both phorbol ester and diacylglycerol. Mutation causes significantly reduced membrane translocation by phorbol ester treatment Mus musculus
Y239A mutated activator-binding site: mutation reduces affinity for both phorbol ester and diacylglycerol. Mutation causes significantly reduced membrane translocation by phorbol ester treatment Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7000
-
 SDS-PAGE, PKCthetaC1B subdomain Mus musculus
100000
-
 SDS-PAGE, full-length PKCtheta Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using affinity purification through glutathione-Sepharose 4B columns, subsequent cleavage of the GST tag, ammonium sulfate precipitation and gel filtration chromatography Mus musculus

Synonyms

Synonyms Comment Organism
PKCthetaC1B C1B subdomain Mus musculus