Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.11.13 extracted from

  • Toita, R.; Mori, T.; Naritomi, Y.; Kang, J.H.; Shiosaki, S.; Niidome, T.; Katayama, Y.
    Fluorometric detection of protein kinase Calpha activity based on phosphorylation-induced dissociation of a polyion complex (2012), Anal. Biochem., 424, 130-136.
    View publication on PubMed

Application

Application Comment Organism
molecular biology a technique is developed to detect PKCalpha activity in a cancerous cell lysate through the simple measurement of fluorescence intensity. The principle of this methodology is based on a fluorescence increase associated with polyion complex dissociation due to phosphorylation by PKCalpha Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Ro-31-8425
-
Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
U-87MG cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alphatomega peptide + ATP an improved PKCalpha-responsive polyion complex (PIC) to measure PKCalpha activity is reported. The polycation molecule is the poly(amidoamine) (PAMAM) dendrimer modified with both cationic PKCalpha-specific peptide substrates (alphatomega, FKKQGSFAKKK-NH2) and near infrared (NIR) fluorophores (Cy5.5), and the polyanion molecule is the quencher (BHQ-3)-modified chondroitin sulfate Homo sapiens ADP + phosphorylated-alphatomega peptide
-
?

Synonyms

Synonyms Comment Organism
PKCalpha
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
assay at Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000008
-
pH 7.3, 25°C Homo sapiens Ro-31-8425