Application | Comment | Organism |
---|---|---|
molecular biology | a technique is developed to detect PKCalpha activity in a cancerous cell lysate through the simple measurement of fluorescence intensity. The principle of this methodology is based on a fluorescence increase associated with polyion complex dissociation due to phosphorylation by PKCalpha | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ro-31-8425 | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
U-87MG cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alphatomega peptide + ATP | an improved PKCalpha-responsive polyion complex (PIC) to measure PKCalpha activity is reported. The polycation molecule is the poly(amidoamine) (PAMAM) dendrimer modified with both cationic PKCalpha-specific peptide substrates (alphatomega, FKKQGSFAKKK-NH2) and near infrared (NIR) fluorophores (Cy5.5), and the polyanion molecule is the quencher (BHQ-3)-modified chondroitin sulfate | Homo sapiens | ADP + phosphorylated-alphatomega peptide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PKCalpha | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
assay at | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000008 | - |
pH 7.3, 25°C | Homo sapiens | Ro-31-8425 |