Activating Compound | Comment | Organism | Structure |
---|---|---|---|
diacylglycerol | binds to the C1 domains of isozymes PKCalpha and PKCgamma, differential roles of C1A and C1B, overview | Rattus norvegicus | |
additional information | activation mechanism of isozymes PKCalpha and PKCgamma | Rattus norvegicus | |
phorbol 12-myristate 13-acetate | binds to the C1 domains of isozymes PKCalpha and PKCgamma, differential roles of C1A and C1B, overview | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
expression of full-length isozymes PKCalpha and PKCgamma in Spodoptera frugiperda Sf9 cells using the baculovirus infection system, expression of His-tagged isolated isozymes PKCalpha and PKCgamma domains C1 in Escherichia coli strain BL21(DE3) | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
D116A | site-directed mutagenesis, isozyme PKCgamma domain C1B mutant shows unaltered activity | Rattus norvegicus |
D55A | site-directed mutagenesis, isozyme PKCalpha and PKCgamma mutants show unaltered activity and ligand binding, isozyme PKCalpha shows enhanced activation by phospholipids | Rattus norvegicus |
W58A | site-directed mutagenesis, isozyme PKCgamma domain C1A mutant shows impaired binding of phorbol 12-myristate 13-acetate and diacylglycerol | Rattus norvegicus |
Y123A | site-directed mutagenesis, isozyme PKCgamma domain C1B mutant shows impaired binding of phorbol 12-myristate 13-acetate and diacylglycerol | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | binding kinetics of phorbol 12-myristate 13-acetate and diacylglycerol | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | cellular membrane translocation mechanism of isozyme PKCalpha and PKCgamma | Rattus norvegicus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Rattus norvegicus | |
Mg2+ | - |
Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
several isozymes | - |
Purification (Comment) | Organism |
---|---|
recombinant full-length isozymes PKCalpha and PKCgamma from Sf9 cells by ion exchange chromatography, recombinant His-tagged isolated isozymes PKCalpha and PKCgamma domains C1 from Escherichia coli strain BL21(DE3) inclusion bodies by nickel affinity chromatography | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + histone III-SS | commercial substrate | Rattus norvegicus | ADP + phosphorylated histone III-SS | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PKC | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus |