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Literature summary for 2.7.11.1 extracted from

  • Sommer, L.; Schaad, M.; Dames, S.
    NMR- and circular dichroism-monitored lipid binding studies suggest a general role for the FATC domain as membrane anchor of phosphatidylinositol 3-kinase-related kinases (PIKK) (2013), J. Biol. Chem., 288, 20046-20063.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
cloning and recombinant expression of the isolated ATM FATC domain in Escherichia coli strain BL21(DE3) fused to the B1 domain of protein G, GB1 Homo sapiens
cloning and recombinant expression of the isolated FATC domain, hDNAPKfatc, in Escherichia coli strain BL21(DE3) as GB1 fusion protein Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasmic vesicle
-
Homo sapiens 31410
-
membrane role for the FATC domain as membrane anchor of phosphatidylinositol 3-kinase-related kinases (PIKK) Homo sapiens 16020
-
nucleus
-
Homo sapiens 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + rapamycin Homo sapiens
-
ADP + phospho-rapamycin
-
?
additional information Homo sapiens the PIKK family members phosphorylate proteins that regulate processes such as DNA repair, cell cycle progression, cellular senescence, and apoptosis ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P78527
-
-
Homo sapiens Q13315
-
-
Homo sapiens Q13535
-
-
Homo sapiens Q96Q15
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GB1-tagged FATC domain, hDNAPKfatc, from Escherichia coli strain BL21(DE3) by immunoaffinity chromatography, tag cleavage Homo sapiens
recombinant isolated GB1-fusion ATM FATC domain from Escherichia coli strain BL21(DE3) by immunoaffinity chromatography, tag cleavage Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation lyophilized FATC domain Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + rapamycin
-
Homo sapiens ADP + phospho-rapamycin
-
?
additional information the PIKK family members phosphorylate proteins that regulate processes such as DNA repair, cell cycle progression, cellular senescence, and apoptosis Homo sapiens ?
-
?
additional information the FATC domain reacts with membrane mimetics, titration analysis, overview Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
More domain organization of PIKKs, overview. The FATC domain is required for protein-protein interactions. Residues 2612-2644 correspond to the FATC domain Homo sapiens
More domain organization of PIKKs, overview. The FATC domain is required for protein-protein interactions. Residues 3024-3056 comprise the FATC domain Homo sapiens
More domain organization of PIKKs, overview. The FATC domain is required for protein-protein interactions. Residues 3629-3661 correspond to the FATC domain Homo sapiens
More domain organization of PIKKs, overview. The FATC domain is required for protein-protein interactions. Residues 4096-4128 correspond to the FATC domain, hDNAPKfatc Homo sapiens

Synonyms

Synonyms Comment Organism
ataxia- and Rad3-related
-
Homo sapiens
ataxia-telangiectasia mutated
-
Homo sapiens
ATM
-
Homo sapiens
ATR
-
Homo sapiens
DNA-dependent protein kinase catalytic subunit
-
Homo sapiens
DNA-PKcs
-
Homo sapiens
phosphatidylinositol 3-kinase-related kinase
-
Homo sapiens
PIKK
-
Homo sapiens
SMG-1
-
Homo sapiens
suppressor of morphogenesis in genitalia-1
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at room temperature Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens

General Information

General Information Comment Organism
evolution the enzyme is a member of the family of phosphatidylinositol-3 kinase-related kinases (PIKKs). All of these can interact with different membrane mimetics and may have different preferences only for membrane properties such as surface charge, curvature, and lipid packing. The FATC domain is shared by all members of the family and plays an important role for the regulation of each PIKK. Sequence conservation of the FATC domain Homo sapiens
additional information characterization and comparisons of the interactions with lipids and different membrane mimetics for the FATC domains of human DNA-PKcs, human ATM, human ATR, human SMG-1, and human TRRAP by NMR and CD spectroscopy, overview Homo sapiens
physiological function the enzyme is important for DNA damage response and signaling in the presence of DNA damages Homo sapiens