Cloned (Comment) | Organism |
---|---|
cloning and recombinant expression of the isolated ATM FATC domain in Escherichia coli strain BL21(DE3) fused to the B1 domain of protein G, GB1 | Homo sapiens |
cloning and recombinant expression of the isolated FATC domain, hDNAPKfatc, in Escherichia coli strain BL21(DE3) as GB1 fusion protein | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasmic vesicle | - |
Homo sapiens | 31410 | - |
membrane | role for the FATC domain as membrane anchor of phosphatidylinositol 3-kinase-related kinases (PIKK) | Homo sapiens | 16020 | - |
nucleus | - |
Homo sapiens | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + rapamycin | Homo sapiens | - |
ADP + phospho-rapamycin | - |
? | |
additional information | Homo sapiens | the PIKK family members phosphorylate proteins that regulate processes such as DNA repair, cell cycle progression, cellular senescence, and apoptosis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P78527 | - |
- |
Homo sapiens | Q13315 | - |
- |
Homo sapiens | Q13535 | - |
- |
Homo sapiens | Q96Q15 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant GB1-tagged FATC domain, hDNAPKfatc, from Escherichia coli strain BL21(DE3) by immunoaffinity chromatography, tag cleavage | Homo sapiens |
recombinant isolated GB1-fusion ATM FATC domain from Escherichia coli strain BL21(DE3) by immunoaffinity chromatography, tag cleavage | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | lyophilized FATC domain | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + rapamycin | - |
Homo sapiens | ADP + phospho-rapamycin | - |
? | |
additional information | the PIKK family members phosphorylate proteins that regulate processes such as DNA repair, cell cycle progression, cellular senescence, and apoptosis | Homo sapiens | ? | - |
? | |
additional information | the FATC domain reacts with membrane mimetics, titration analysis, overview | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | domain organization of PIKKs, overview. The FATC domain is required for protein-protein interactions. Residues 2612-2644 correspond to the FATC domain | Homo sapiens |
More | domain organization of PIKKs, overview. The FATC domain is required for protein-protein interactions. Residues 3024-3056 comprise the FATC domain | Homo sapiens |
More | domain organization of PIKKs, overview. The FATC domain is required for protein-protein interactions. Residues 3629-3661 correspond to the FATC domain | Homo sapiens |
More | domain organization of PIKKs, overview. The FATC domain is required for protein-protein interactions. Residues 4096-4128 correspond to the FATC domain, hDNAPKfatc | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
ataxia- and Rad3-related | - |
Homo sapiens |
ataxia-telangiectasia mutated | - |
Homo sapiens |
ATM | - |
Homo sapiens |
ATR | - |
Homo sapiens |
DNA-dependent protein kinase catalytic subunit | - |
Homo sapiens |
DNA-PKcs | - |
Homo sapiens |
phosphatidylinositol 3-kinase-related kinase | - |
Homo sapiens |
PIKK | - |
Homo sapiens |
SMG-1 | - |
Homo sapiens |
suppressor of morphogenesis in genitalia-1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the family of phosphatidylinositol-3 kinase-related kinases (PIKKs). All of these can interact with different membrane mimetics and may have different preferences only for membrane properties such as surface charge, curvature, and lipid packing. The FATC domain is shared by all members of the family and plays an important role for the regulation of each PIKK. Sequence conservation of the FATC domain | Homo sapiens |
additional information | characterization and comparisons of the interactions with lipids and different membrane mimetics for the FATC domains of human DNA-PKcs, human ATM, human ATR, human SMG-1, and human TRRAP by NMR and CD spectroscopy, overview | Homo sapiens |
physiological function | the enzyme is important for DNA damage response and signaling in the presence of DNA damages | Homo sapiens |