Application | Comment | Organism |
---|---|---|
medicine | spleen tyrosine kinase is involved in the activation of cells implicated in allergic or autoimmune diseases and certain cancers, inhibitors may prove to be effective in treating diseases where Syk activity is increased or deregulated | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
full-length Syk is cloned into pFastBac HTb with an N-terminal 6-histidine tag and rTEV protease cleavage site, Syk protein is expressed in SF9 insect cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
BAY61-3606 | - |
Homo sapiens | |
R112 | - |
Homo sapiens | |
R406 | - |
Homo sapiens | |
YM193306 | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
ATP | - |
Homo sapiens | |
0.057 | - |
EEEEYIQ[dP]-8-hydroxy-5-(N,N-dimethylsulfonamido)-2-methylquinoline-G | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | preactivation of the enzyme by incubation in the presence of 8.3 mM ATP and 16.6 mM MgCl2 | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [protein]-L-tyrosine | Homo sapiens | - |
ADP + [protein]-L-tyrosine phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using a Ni-NTA Superflow and a Source 15Q column | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Ramos cell | - |
Homo sapiens | - |
Storage Stability | Organism |
---|---|
-80°C, 20 mM MES buffer, pH 6.0, 200 mM NaCl, 10 mM betaMcEtOH, 10% glycerol, 1 mM benzamidine, 0.2 mM PMSF | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + EEEEYIQ[dP]-8-hydroxy-5-(N,N-dimethylsulfonamido)-2-methylquinoline-G | Y7 Sox-based substrate, kinetic assay | Homo sapiens | ADP + EEEEYIQ[dP]-8-hydroxy-5-(N,N-dimethylsulfonamido)-2-methylquinoline-G phosphate | - |
? | |
ATP + [protein]-L-tyrosine | - |
Homo sapiens | ADP + [protein]-L-tyrosine phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Spleen tyrosine kinase | - |
Homo sapiens |
Syk | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
55 | - |
ATP | - |
Homo sapiens | |
396 | - |
EEEEYIQ[dP]-8-hydroxy-5-(N,N-dimethylsulfonamido)-2-methylquinoline-G | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | preactivation of the enzyme by incubation in the presence of 8.3 mM ATP and 16.6 mM MgCl2 | Homo sapiens |