Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | constitutive SFK activation is associated with tumorigenesis, Hck and Lyn perform autophosphorylation for activation | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of Hck mutant K267M and Lyn mutant K274M in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K267M | a Hck mutant, the inability of HckK267M to undergo autophosphorylation promotes its phosphorylation at YT by CSK to a higher extent than that of wild type Hck | Homo sapiens |
K274M | Lyn mutant, Lyn K274M does not form a stable protein complex with CHK, whereas its unphosphorylated counterpart does | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
C-terminal Src kinase | i.e. CSK, an inhibitor which inactivates Src family of protein kinases by phosphorylating a consensus tyrosine YT near the C terminus, phosphorylated YT then intramolecularly binds to the enzyme's SH2 domain, pYT/SH2 interaction, together with binding between the SH2 kinase linker and the SH3 domain, stabilizes the enzyme in a closed inactive conformation, preparation of recombinant SH2 and SH3 domains by expression in Escherichia coli, overview | Homo sapiens | |
CSK-homologous kinase | i.e. CHK, an inhibitor which inactivates Src family of protein kinases by phosphorylating a consensus tyrosine YT near the C terminus, phosphorylated YT then intramolecularly binds to the enzyme's SH2 domain, pYT/SH2 interaction, together with binding between the SH2 kinase linker and the SH3 domain, stabilizes the enzyme in a closed inactive conformation, a second non-catalytic inhibitory mechanism involves tight binding of CHK to the enzyme's SFKs. The binding alone is sufficient to inhibit SFKs inhibition, preparation of recombinant SH2 and SH3 domains by expression in Escherichia coli, overview | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens | |
Mn2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Src family kinase | Homo sapiens | substrate of the protein kinases CSK and CHK, which specifically phosphorylate a tyrosine residue at the C-terminus forming intramolecular bonds to the SH2 domain and inhibiting the Src family kinase, overview | ? | - |
? | |
additional information | Homo sapiens | the Src family of protein kinases mediates mitogenic signal transduction, and constitutive activation of the enzymes is associated with tumorigenesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | Hck and Lyn perform autophosphorylation for activation | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant Lyn mutant K274M from Spodoptera frugiperda Sf9 cells by anion exchange and hydroxyapatite chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + cdc2(6-20) peptide | i.e. KVEKIGEGTYGVVYK, substrate of Src family kinases | Homo sapiens | ? | - |
? | |
ATP + Src family kinase | substrate of the protein kinases CSK and CHK, which specifically phosphorylate a tyrosine residue at the C-terminus forming intramolecular bonds to the SH2 domain and inhibiting the Src family kinase, overview | Homo sapiens | ? | - |
? | |
additional information | the Src family of protein kinases mediates mitogenic signal transduction, and constitutive activation of the enzymes is associated with tumorigenesis | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
C-terminal Src kinase | - |
Homo sapiens |
CHK | - |
Homo sapiens |
Csk | - |
Homo sapiens |
CSK-homologous kinase | - |
Homo sapiens |
Lyn | - |
Homo sapiens |
SFK | - |
Homo sapiens |
Src family kinase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |