Literature summary for 2.7.10.2 extracted from

Gnemmi, I.; Scotti, C.; Cappelletti, D.; Canonico, P.L.; Condorelli, F.; Rosano, C.
Expression, purification and preliminary crystallographic studies on the catalytic region of the nonreceptor tyrosine kinase Fes (2007), Acta Crystallogr. Sect. F, 63, 18-20.

Cloned(Commentary)

Cloned (Comment)	Organism
feline sarcoma oncogene, c-fps/fes, located at chromosome 15q25-qter, expression of a 281 amino acid sequence fragment as His-Fes-CR-FLAG in Escherichia coli strain M15pREP4	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His- and FLAG-tagged catalytic domain of Fes, hanging drop vapour diffusion method, 0.002 ml of 10 mg/ml protein solution, phosphate buffered saline, pH 7.4, is mixed with 0.002 ml reservoir solution and equilibrated against 0.4 ml reservoir solution containing 1.6 M magnesium sulfate and 0.1 M MES pH 6.5, 27°C, X-ray diffraction structure determination and analysis at 3.9 A resolution	Homo sapiens

Crystallization (Comment)

Organism

purified recombinant His- and FLAG-tagged catalytic domain of Fes, hanging drop vapour diffusion method, 0.002 ml of 10 mg/ml protein solution, phosphate buffered saline, pH 7.4, is mixed with 0.002 ml reservoir solution and equilibrated against 0.4 ml reservoir solution containing 1.6 M magnesium sulfate and 0.1 M MES pH 6.5, 27°C, X-ray diffraction structure determination and analysis at 3.9 A resolution

Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	Fes is involved in the regulation of cell-cell and cell-matrix interactions mediated by adherens junctions and focal adhesions, the activated form of the kinase can induce cellular transformation, overview	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P07332	gene c-fps/fes, the feline sarcoma oncogene	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His- and FLAG-tagged catalytic domain of Fes from Escherichia coli strain M15 by nickel affinity chromatography and diaflitration	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
marrow cell	-	Homo sapiens	-
neuron	-	Homo sapiens	-
vascular endothelial cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	Fes is involved in the regulation of cell-cell and cell-matrix interactions mediated by adherens junctions and focal adhesions, the activated form of the kinase can induce cellular transformation, overview	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
More	the N-terminus of Fes contains the FCH, i.e. Fps/Fes/Fer/CIP4 homology, domain, which is unique to the Fes/Fer kinase family, followed by three coiled-coil domains and an SH2, i.e. Src-homology 2, domain, the catalytic region Fes-CR is located at the C-terminus of the protein	Homo sapiens

Synonyms

Synonyms	Comment	Organism
More	the enzyme belongs to the nonreceptor protein-tyrosine kinase, PTK, family	Homo sapiens
nonreceptor tyrosine kinase Fes	-	Homo sapiens