Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | Csk is tyrosine autophosphorylated in the activation loop, structure | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant His-tagged Csk in Escherichia coli strain BL21(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D332A | site-directed mutagenesis of Csk in the DFG motif in front of the activation loop leads to complete loss of kinase activity | Homo sapiens |
D344A | site-directed mutagenesis of Csk in the activation loop, mutant shows slightly increased activity with kdSrc and slightly reduced activity with poly(Glu4-Tyr) as substrates compared to the wild-type enzyme | Homo sapiens |
E338A | site-directed mutagenesis of Csk in the activation loop, mutant shows 40-60% remaining activity compared to the wild-type enzyme | Homo sapiens |
G346A | site-directed mutagenesis of Csk in the activation loop, mutant shows decreased activity compared to the wild-type enzyme | Homo sapiens |
K337A | site-directed mutagenesis of Csk in the activation loop, mutant shows slightly decreased activity compared to the wild-type enzyme | Homo sapiens |
additional information | construction of a residue-339-deletion mutant | Homo sapiens |
Q343A | site-directed mutagenesis of Csk in the activation loop, mutant shows slightly decreased activity compared to the wild-type enzyme | Homo sapiens |
R313A | site-directed mutagenesis, activity similar to the wild-type enzyme | Homo sapiens |
S340A | site-directed mutagenesis of Csk in the activation loop, mutant shows slightly decreased activity compared to the wild-type enzyme | Homo sapiens |
S341A | site-directed mutagenesis of Csk in the activation loop, mutant shows slightly decreased activity compared to the wild-type enzyme | Homo sapiens |
T336A | site-directed mutagenesis of Csk in the activation loop, mutant shows increased activity compared to the wild-type enzyme | Homo sapiens |
T342A | site-directed mutagenesis of Csk in the activation loop, mutant shows decreased activity compared to the wild-type enzyme | Homo sapiens |
T345A | site-directed mutagenesis of Csk in the activation loop, mutant shows decreased activity compared to the wild-type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
[kdSrc kinase]-L-tyrosine | pH 8.0, 30°C, recombinant mutant E338A Csk | Homo sapiens | |
0.01 | - |
[kdSrc kinase]-L-tyrosine | pH 8.0, 30°C, recombinant wild-type Csk | Homo sapiens | |
0.24 | - |
[kdSrc kinase]-L-tyrosine | pH 8.0, 30°C, recombinant Csk residue-339-deletion mutant | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | Csk is tyrosine autophosphorylated in the activation loop | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant His-tagged Csk from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + poly(Glu4-Tyr) | - |
Homo sapiens | ADP + poly(Glu4-Tyr)-L-tyrosine phosphate | - |
? | |
ATP + [kdSrc kinase]-L-tyrosine | i.e. kinase-defective chicken Src mutant K295M | Homo sapiens | ADP + [kdSrc kinase]-L-tyrosine phosphate | - |
? | |
additional information | the activation loop is not essential for enzyme activity | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Csk protein-tyrosine kinase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
poly(Glu4-Tyr) | pH 8.0, 30°C, recombinant Csk residue-339-deletion mutant | Homo sapiens | |
0.1 | - |
poly(Glu4-Tyr) | pH 8.0, 30°C, recombinant mutant E338A Csk | Homo sapiens | |
0.35 | - |
[kdSrc kinase]-L-tyrosine | pH 8.0, 30°C, recombinant Csk residue-339-deletion mutant | Homo sapiens | |
0.57 | - |
poly(Glu4-Tyr) | pH 8.0, 30°C, recombinant wild-type Csk | Homo sapiens | |
0.82 | - |
[kdSrc kinase]-L-tyrosine | pH 8.0, 30°C, recombinant mutant E338A Csk | Homo sapiens | |
1.88 | - |
[kdSrc kinase]-L-tyrosine | pH 8.0, 30°C, recombinant wild-type Csk | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |