Cloned (Comment) | Organism |
---|---|
catalytic domain Src including the C-terminal tail Src-CD, expressed in Schizosaccharomyces pombe | Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | completely inhibited by an excess of substrate peptide | Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the overall activity kcat of the catalytic domain Src, Src-CD, including the C-terminal tail for two exogenous substrates, the Src substrate peptide AEEEIYGEFEAKKKK and denatured enolase is about 10 times higher than that of wild-type Src. The kcat values for phosphorylation of the Src substrate peptide are similar for the unphosphorylated and monophosphorylated Src-CD, 50 min-1, but the apparent Km values differ significantly, approximately 0.003 mM and 0.010 mM, respectively | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | P00523 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | protein expressed in Schizosaccharomyces pombe is a mixture of unphosphorylated, 80%, and mono-phosphorylated, 20%, species. The mono-phosphorylated form is phosphorylated either at Tyr416 or at Tyr436 | Gallus gallus |
Purification (Comment) | Organism |
---|---|
catalytic domain Src including the C-terminal tail Src-CD, expressed in Schizosaccharomyces pombe | Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
AEEEIYGEFEAKKKK + ATP | - |
Gallus gallus | ? + ADP | - |
? | |
denatured enolase + ATP | - |
Gallus gallus | phosphorylated denatured enolase + ADP | - |
? | |
additional information | catalytic domain Src including the C-terminal tail autophosphorylates and efficiently phosphorylates substrate peptides and proteins. Autophosphorylation occurs by an intermolecular mechanism | Gallus gallus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
proto-oncogene tyrosine-protein kinase SRC | - |
Gallus gallus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the overall activity kcat of the catalytic domain Src, Src-CD, including the C-terminal tail for two exogenous substrates, the Src substrate peptide AEEEIYGEFEAKKKK and denatured enolase is about 10 times higher than that of wild-type Src. The kcat values for phosphorylation of the Src substrate peptide are similar for the unphosphorylated and monophosphorylated Src-CD, 50 min-1 | Gallus gallus |