analysis |
proximity ligation assay-based methodology for in situ visualization and quantification of ligand-dependent EGFR receptor dimerization in intact cells. Using the approach combined with a universally applicable epitope tagging strategy, EGFR dimers can be detect in cells transiently co-expressing FLAG-tagged and MYC-tagged human EGFRs. Data strongly suggest that the method can be used to detect ligand-dependent EGFR dimerization, and the signal is generated in a protein interaction-based manner |
Homo sapiens |