Cloned (Comment) | Organism |
---|---|
expression of EGFR-TK in Spodoptera frugiperda Sf9 cells using the baculovirus infection system | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
gefitinib | specific inhibition of EGFR-TK | Homo sapiens | |
PD153035 | specific inhibition of EGFR-TK, IC50 is 0.00329 mM with 1 U of purified EGFR-TK from A431 cells | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of wild-type EGFR-TK from A431 cells and recombinant EGFR-TK from Sf9 cells | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a [protein]-L-tyrosine | Homo sapiens | - |
ADP + a [protein]-L-tyrosine phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
EGFR-TK from A431 cells by affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A-431 cell | - |
Homo sapiens | - |
brain tumor cell | overexpression of EGFR-TK | Homo sapiens | - |
breast cancer cell | overexpression of EGFR-TK | Homo sapiens | - |
colonic cancer cell | overexpression of EGFR-TK | Homo sapiens | - |
lung cancer cell | overexpression of EGFR-TK | Homo sapiens | - |
ovarian cancer cell | overexpression of EGFR-TK | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
development of a specific ELISA-based phosphotyrosine detection | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a [protein]-L-tyrosine | - |
Homo sapiens | ADP + a [protein]-L-tyrosine phosphate | - |
? | |
ATP + [poly-(Glu,Tyr)1:4] | synthetic substrate | Homo sapiens | ADP + [poly-(Glu,Tyr)1:4]-tyrosine phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EGFR tyrosine kinase | - |
Homo sapiens |
EGFR-TK | - |
Homo sapiens |
epidermal growth factor receptor tyrosine kinase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00329 | - |
specific inhibition of EGFR-TK, IC50 is 0.00329 mM with 1 U of purified EGFR-TK from A431 cells | Homo sapiens | PD153035 |