Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | activation mechanism | eukaryota |
Crystallization (Comment) | Organism |
---|---|
X-ray diffraction structure analysis of InRK and of FGFR with phosphorylated activation loop embedded in the substrate pocket | eukaryota |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | InRK: random kinetic mechanism, reaction kinetic can be influenced by the sort of substrate | eukaryota |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
eukaryota | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | can partially substitue Mg2+ | eukaryota | |
Co2+ | can partially substitue Mg2+ | eukaryota | |
Mg2+ | - |
eukaryota | |
Mg2+ | dependent on, Mg2+ is the physiologic metal ion, other divalent cations are able to support nucleotide binding, but only Mn2+, Co2+, and Cd2+ can substitute Mg2+ in supporting the catalytic activity | eukaryota | |
Mn2+ | can partially substitue Mg2+ | eukaryota |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a protein | eukaryota | - |
ADP + a phosphoprotein | - |
? | |
additional information | eukaryota | mutations in the glycine-riche loop of MET can cause papillary renal-cell carcinomas | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
eukaryota | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phosphorylation regulates the enzyme activity, but has only a small influence on substrate binding by InRK, and no influence on substrate binding of Tie-2 | eukaryota |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate | reaction mechanism | eukaryota | |
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate | regulation of enzyme activity involves the activation loop, a polypeptide region outside the active site cleft, which is reversibly phosphorylated | eukaryota |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a protein | - |
eukaryota | ADP + a phosphoprotein | - |
? | |
additional information | mutations in the glycine-riche loop of MET can cause papillary renal-cell carcinomas | eukaryota | ? | - |
? | |
additional information | poor activity on free amino acids, consensus sequence of InRK is YM-MM, and of EGFR E-EEYF | eukaryota | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Egfr | - |
eukaryota |
epidermal growth factor receptor | - |
eukaryota |
FGFR | - |
eukaryota |
fibroblast growth factor receptor | - |
eukaryota |
InRK | - |
eukaryota |
insulin receptor kinase | - |
eukaryota |
MET | - |
eukaryota |
receptor PTK | - |
eukaryota |
Tie-2 | - |
eukaryota |
tyrosine kinase domain of the insulin receptor | - |
eukaryota |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | eukaryota | |
ATP | the binding site is a deep pocket lined by hydrophobic residues, enzyme affinity of Tie-2 for ATP is not influenced by phosphorylation of the activation loop | eukaryota |