Literature summary for 2.7.1.91 extracted from

Meacci, E.; Nuti, F.; Donati, C.; Cencetti, F.; Farnararo, M.; Bruni, P.
Sphingosine kinase activity is required for myogenic differentiation of C2C12 myoblasts (2008), J. Cell. Physiol., 214, 210-220.

Search for more literature for 2.7.1.91

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in C2C12 cell	Mus musculus

Protein Variants

Protein Variants	Comment	Organism
G82D	catalytically inactive. Overexpression in myoblast cells significantly increases cell growth and delays the beginning of myogenesis	Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
C2C12 cell	-	Mus musculus	-

General Information

General Information	Comment	Organism
physiological function	SphK activity, SphK1 protein content and sphingosine 1-phosphate formation are enhanced in myoblasts that became confluent as well as in differentiating cells. Enforced expression of SphK1 reduces the myoblast proliferation rate, enhances the expression of myogenic differentiation markers and anticipates the onset of differentiated muscle phenotype. Down-regulation of SphK1 by specific silencing byRNA interference or overexpression of a catalytically inactive SphK1, significantly increases cell growth and delays the beginning of myogenesis. Exogenous addition of sphingosine 1-phosphate rescues the biological processes. Stimulation of myogenesis in SphK1-overexpressing myoblasts is abrogated by treatment with short interfering RNA specific for S1P2 receptor	Mus musculus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)