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Literature summary for 2.7.1.91 extracted from

  • Don, A.S.; Rosen, H.
    A lipid binding domain in sphingosine kinase 2 (2009), Biochem. Biophys. Res. Commun., 380, 87-92.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
cholesterol 0.01 mM, isoform SphK2, 107% of initial activity Homo sapiens
galactosylceramide 0.01 mM, isoform SphK2, 84% of initial activity, isoform SphK1, 109% of initial activity Homo sapiens
galactosylceramide 3-sulfate 0.01 mM, isoform SphK2, 37% of initial activity, isoform SphK1, 156% of initial activity Homo sapiens
phosphatidic acid 0.01 mM, isoform SphK2, 102% of initial activity, isoform SphK1, 265% of initial activity Homo sapiens
phosphatidylinositol 0.01 mM, isoform SphK2,187% of initial activity Homo sapiens
phosphatidylinositol 4-phosphate 0.01 mM, isoform SphK2, 89% of initial activity, isoform SphK1, 175% of initial activity Homo sapiens
phosphatidylserine 0.01 mM, isoform SphK2, 203% of initial activity Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
-
Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information isoform SphK2 contains a lipid binding domain at the N-terminal residues 1-175, a region of sequence that is absent in Sphk1. Deleting the N-terminal domain reduces Sphk2 membrane localisation in cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
3-O-sulfogalactosylceramide endogenous glycolipid sulfatide, binds to and inhibits the activity of isoform Sphk2 and the closely related ceramide kinase Cerk, but not isoform Sphk1. The lipid binding domain is mapped to the N-terminus of Sphk2, residues 1-175, a region of sequence that is absent in Sphk1, but aligns with a pleckstrin homology domain in Cerk Homo sapiens
galactosylceramide 0.01 mM, isoform SphK2, 84% of initial activity, isoform SphK1, 109% of initial activity Homo sapiens
galactosylceramide 3-sulphate 0.01 mM, isoform SphK2, 37% of initial activity, isoform SphK1, 156% of initial activity Homo sapiens
additional information isoform Sphk2 binds to phosphatidylinositol monophosphates but not to abundant cellular phospholipids Homo sapiens
phosphatidylinositol 4,5-bisphosphate 0.01 mM, isoform SphK2, 60% of initial activity, isoform SphK1, 90% of initial activity Homo sapiens
phosphatidylinositol 4-phosphate 0.01 mM, isoform SphK2, 89% of initial activity, isoform SphK1, 175% of initial activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
nitrobenzoxadiazole-labeled sphingosine pH 7.4, 35°C, presence of 10 microM 3-O-sulfogalactosylceramide Homo sapiens
1.7
-
nitrobenzoxadiazole-labeled sphingosine pH 7.4, 35°C Homo sapiens
1.7
-
nitrobenzoxadiazole-labeled sphingosine pH 7.4, 35°C, presence of 25 microM 3-O-sulfogalactosylceramide Homo sapiens
3.6
-
nitrobenzoxadiazole-labeled sphingosine pH 7.4, 35°C, presence of 4 microM 3-O-sulfogalactosylceramide Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane deleting the N-terminal domain with residues 1-175 reduces Sphk2 membrane localisation in cells Homo sapiens 16020
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-D-erythro-sphingosine
-
Homo sapiens ADP + N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-D-erythro-sphingosine 1-phosphate
-
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